{"platform": 4, "owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSE13762GSM346553": {"time": 0, "dose": "  ", "cell type": "CD14-positive monocyte", "compound": "none"}}, {"GSE13762GSM346557": {"time": 5, "dose": "  ", "cell type": "immature dendritic cell", "compound": "vehicle"}}, {"GSE13762GSM346558": {"time": 5, "dose": "10", "cell type": "immature dendritic cell", "compound": "1,25-dihydroxyvitamin D3 "}}, {"GSE13762GSM346559": {"time": 12, "dose": "  ", "cell type": "differentiating dendritic cell", "compound": "vehicle"}}, {"GSE13762GSM346562": {"time": 12, "dose": "10", "cell type": "differentiating dendritic cell", "compound": "1,25-dihydroxyvitamin D3 "}}, {"GSE13762GSM346553": {"time": 0, "dose": "  ", "cell type": "CD14-positive monocyte", "compound": "none"}}, {"GSE13762GSM346557": {"time": 5, "dose": "  ", "cell type": "immature dendritic cell", "compound": "vehicle"}}, {"GSE13762GSM346558": {"time": 5, "dose": "10", "cell type": "immature dendritic cell", "compound": "1,25-dihydroxyvitamin D3 "}}, {"GSE13762GSM346559": {"time": 12, "dose": "  ", "cell type": "differentiating dendritic cell", "compound": "vehicle"}}, {"GSE13762GSM346562": {"time": 12, "dose": "10", "cell type": "differentiating dendritic cell", "compound": "1,25-dihydroxyvitamin D3 "}}, {"GSE13762GSM346553": {"time": 0, "dose": "  ", "cell type": "CD14-positive monocyte", "compound": "none"}}, {"GSE13762GSM346557": {"time": 5, "dose": "  ", "cell type": "immature dendritic cell", "compound": "vehicle"}}, {"GSE13762GSM346558": {"time": 5, "dose": "10", "cell type": "immature dendritic cell", "compound": "1,25-dihydroxyvitamin D3 "}}, {"GSE13762GSM346559": {"time": 12, "dose": "  ", "cell type": "differentiating dendritic cell", "compound": "vehicle"}}, {"GSE13762GSM346562": {"time": 12, "dose": "10", "cell type": "differentiating dendritic cell", "compound": "1,25-dihydroxyvitamin D3 "}}], "id": 3103, "ownerprofile_id": "arrayexpress_sid", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13762", "summary_wrapped": "We have carried out global gene expression analysis to clarify the interrelationship between 1,25-dihydroxyvitamin D3 and...", "pubmed_id": 19201860, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 4, "sample_count": 15, "tags": ["cell", "dendritic", "genome", "monocyte"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-by-array-of-human-monocy-3", "geo_id_plat": "E-GEOD-13762_A-AFFY-44", "name": "Transcription profiling by array of human monocyte-derived dendritic cells treated with 1,25-dihydroxyvitamin D3", "created": "Sep.11, 2014", "summary": "We have carried out global gene expression analysis to clarify the interrelationship between 1,25-dihydroxyvitamin D3 and differentiation-driven gene expression patterns in developing human monocyte-derived dendritic cells. Monocytes were treated with 10 nM 1,25-dihydroxyvitamin D3 or vehicle 14 hours after plating for 12 hours or 5 days. Monocytes, differentiating dendritic cells (+/-1,25-dihydroxyvitamin D3 for 12 hours) and immature dendritic cells (+/-1,25-dihydroxyvitamin D3 for 5 days) were harvested. This design allows one to identify genes regulated by differentiation and/or 1,25-dihydroxyvitamin D3 in human monocyte-derived dendritic cells. Experiment Overall Design: Human monocytes were obtained from buffy coats from healthy donors by Ficoll gradient centrifugation followed by immunomagnetic cell separation with anti-CD14-conjugated microbeads. Monocytes were cultured in RPMI-1640 supplemented with 10% FBS, 800 U/ml GM-CSF and 500 U/ml IL-4. Monocytes were treated with 10 nM 1,25-dihydroxyvitamin D3 or vehicle 14 hours after plating for 12 hours or 5 days. Monocytes, differentiating dendritic cells (+/-1,25-dihydroxyvitamin D3 for 12 hours) and immature dendritic cells (+/-1,25-dihydroxyvitamin D3 for 5 days) were harvested. Experiments were performed in biological triplicates representing samples from different donors. 15 samples were processed and hybridized to Human Genome U133 Plus 2.0 Arrays.", "geo_gse_id": "E-GEOD-13762", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13762/samples/"}