{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 5552, "factors": [{"GSM257459 1": {}}, {"GSM257460 1": {}}, {"GSM257461 1": {}}, {"GSM257462 1": {}}, {"GSM257464 1": {}}, {"GSM257466 1": {}}, {"GSM257467 1": {}}, {"GSM341443 1": {}}, {"GSM341444 1": {}}, {"GSM341445 1": {}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Conjugated linoleic acid (CLA), a class of fatty acids found in beef and dairy products, has been shown to inhibit tumorigenesis in a...", "pubmed_id": 20624750, "geo_gse_id": "E-GEOD-13553", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 10, "tags": ["breast", "breast cancer", "cancer", "cell", "class", "gas", "genome", "gland", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "the-effect-of-dietary-cla-on-mammary-tumorigenesis", "geo_id_plat": "E-GEOD-13553_A-AFFY-45", "name": "The effect of dietary CLA on mammary tumorigenesis", "created": "Nov.10, 2014", "summary": "Conjugated linoleic acid (CLA), a class of fatty acids found in beef and dairy products, has been shown to inhibit tumorigenesis in a variety of cancer model systems. Based on previously well-documented anti-tumor activity of CLA in rodent models of breast cancer, a pilot study was initiated to examine the effect of dietary CLA in a well-established transgenic model of breast cancer.  Western blots were performed for the detection of AKT, c-Src, ERK1/2, and Cdc24. CLA significantly increased tumor burden (p<0.1) independent of an increase in oncogenic signaling. Mammary gland whole mounts indicated a loss of mammary adipose and extensive epithelial expansion in CLA-treated animals. Microarray analysis indicated a significant reduction in cytoskeletal related genes with at least a two-fold decrease in five out of six CLA-fed animals compared to untreated controls. Reduction of Cdc42, a key regulator of cell adhesion and cytoskeletal arrangements, was confirmed at the protein level by western blot (p<0.01). These findings suggest that dietary CLA may advance the malignant phenotype by promoting a loss of cell polarity and adhesion in the mammary gland epithelium. This action may have serious clinical implications for a subset high-risk population and warrants further investigation. Virgin, four-week-old PyV-mT mice were administered a diet of a mixed-isomer CLA formulation (1% wt/wt) (N=6) or control AIN96G diet (N=5) for four weeks. Measurements of food disappearance, weights and palpations were recorded weekly. All animals were euthanized at eight weeks of age.  Formalin-fixed, paraffin-embedded mammary gland tissue was used for H&E and trichrome staining and immunohistochemistry for Ki67. Tissue levels of CLA were measured by gas chromatography. Thoracic mammary glands were fixed in glacial acetic acid:ethanol and carmine stained. cDNA microarray was performed on RNA from 6 CLA-fed mice and 4 control mice using the Affymetrix 430 2.0 mouse genome chips.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13553", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13553/samples/"}