ArrayExpress Uploader01466arrayexpress_sid3To determine how aging impacts gene expression in hematopoietic stem cells (HSCs), human CD34+ cells from bone marrow (34BM) and...18596738E-GEOD-13496/profile/8773/arrayexpressuploader030bonebone marrowcellstem cellDec.12, 2014Falsetranscription-profiling-of-human-aging-hematopoietE-GEOD-13496_A-AFFY-33Transcription profiling of human aging hematopoietic progenitor/stem cellsJun.19, 2014To determine how aging impacts gene expression in hematopoietic stem cells (HSCs), human CD34+ cells from bone marrow (34BM) and mobilized stem cell products (34P38NPBSC) were examined using microarray-based expression profiling. Differential expression changes were confirmed by microarray comparisons of younger and older expanded T-cell populations. Experiment Overall Design: We compared gene expression profiles between younger and older human CD34+ cells from bone marrow (34BM) and CD34+CD38- cells from mobilized stem cell products(34P38NPBSC). We also examined gene expression changes associated with aging in human expanded T-cells. For 34BM and T-cells, biotin-labeling of total RNA (5 µg) was performed as per standard Affymetrix protocol. For the 34P38NPBSC arrays, the single-stranded linear amplification protocol was used to process total RNA (100 nanograms) for the microarrays. Both protocols are described in detail in PMID14706461.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13496humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13496/samples/