{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 1466, "factors": [{"GSE13496GSM340003": {}}, {"GSE13496GSM339991": {}}, {"GSE13496GSM340012": {}}, {"GSE13496GSM339956": {}}, {"GSE13496GSM339959": {}}, {"GSE13496GSM340005": {}}, {"GSE13496GSM340000": {}}, {"GSE13496GSM339997": {}}, {"GSE13496GSM340010": {}}, {"GSE13496GSM339958": {}}, {"GSE13496GSM340002": {}}, {"GSE13496GSM340008": {}}, {"GSE13496GSM339999": {}}, {"GSE13496GSM339952": {}}, {"GSE13496GSM339990": {}}, {"GSE13496GSM339908": {}}, {"GSE13496GSM339957": {}}, {"GSE13496GSM340004": {}}, {"GSE13496GSM340011": {}}, {"GSE13496GSM339948": {}}, {"GSE13496GSM340009": {}}, {"GSE13496GSM340013": {}}, {"GSE13496GSM340015": {}}, {"GSE13496GSM340007": {}}, {"GSE13496GSM339993": {}}, {"GSE13496GSM340001": {}}, {"GSE13496GSM339998": {}}, {"GSE13496GSM340014": {}}, {"GSE13496GSM339992": {}}, {"GSE13496GSM340006": {}}], "ownerprofile_id": "arrayexpress_sid", "platform": 3, "summary_wrapped": "To determine how aging impacts gene expression in hematopoietic stem cells (HSCs), human CD34+ cells from bone marrow (34BM) and...", "pubmed_id": 18596738, "geo_gse_id": "E-GEOD-13496", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 30, "tags": ["bone", "bone marrow", "cell", "stem cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-human-aging-hematopoiet", "geo_id_plat": "E-GEOD-13496_A-AFFY-33", "name": "Transcription profiling of human aging hematopoietic progenitor/stem cells", "created": "Jun.19, 2014", "summary": "To determine how aging impacts gene expression in hematopoietic stem cells (HSCs), human CD34+ cells from bone marrow (34BM) and mobilized stem cell products (34P38NPBSC) were examined using microarray-based expression profiling. Differential expression changes were confirmed by microarray comparisons of younger and older expanded T-cell populations. Experiment Overall Design: We compared gene expression profiles between younger and older human CD34+ cells from bone marrow (34BM) and CD34+CD38- cells from mobilized stem cell products(34P38NPBSC). We also examined gene expression changes associated with aging in human expanded T-cells. For 34BM and T-cells, biotin-labeling of total RNA (5 \u00b5g) was performed as per standard Affymetrix protocol.  For the 34P38NPBSC arrays, the single-stranded linear amplification protocol was used to process total RNA (100 nanograms) for the microarrays.  Both protocols are described in detail in PMID14706461.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13496", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13496/samples/"}