ArrayExpress Uploader03079wild typeRAF inhibitor #1wild typeRAF inhibitor #1sh2B-raf (doxycycline inducible)RAF inhibitor #1sh2B-raf (doxycycline inducible)RAF inhibitor #1shGFPRAF inhibitor #2shB-Raf (doxycycline inducible)RAF inhibitor #1wild typeBAY-43-9006wild typeBAY-43-9006sh2B-raf (doxycycline inducible)BAY-43-9006sh2B-raf (doxycycline inducible)BAY-43-9006shGFPnoneshB-Raf (doxycycline inducible)nonesh2B-raf (doxycycline inducible)noneshGFPnoneshB-Raf (doxycycline inducible)nonesh2B-raf (doxycycline inducible)noneshGFPnoneshB-Raf (doxycycline inducible)nonewild typeDMSOsh2B-raf (doxycycline inducible)DMSOwild typeDMSOsh2B-raf (doxycycline inducible)DMSOshGFPdoxycyclineshB-Raf (doxycycline inducible)doxycyclinesh2B-raf (doxycycline inducible)doxycyclineshGFPdoxycyclineshB-Raf (doxycycline inducible)doxycyclinesh2B-raf (doxycycline inducible)doxycyclineshGFPdoxycyclineshB-Raf (doxycycline inducible)doxycyclinearrayexpress_sid4Unsupervised hierarchical clustering revealed a strong similarity in gene modulation resulting from either compound treatment or BRAF...19276360E-GEOD-13487/profile/8773/arrayexpressuploader230melanomaDec.12, 2014Falsetranscription-profiling-by-array-of-human-melano-3E-GEOD-13487_A-AFFY-44Transcription profiling by array of human melanoma cells with doxycycline-inducible RNAi-mediated knockdown of B-RafSep.11, 2014Unsupervised hierarchical clustering revealed a strong similarity in gene modulation resulting from either compound treatment or BRAF ablation mediated by RNA interference relative to DMSO-treated control samples . Experiment Overall Design: We have generated melanoma A375 cells stably expressing a shRNA construct for doxycycline-inducible knockdown of B-Raf (2 mg/ml Dox, 48h). Small molecule treatment was at 1000nM R341787, 24h.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13487humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13487/samples/