Dataset: Transcription profiling by array of human melanoma cells with doxycycline-inducible RNAi-mediated knockdown of B-Raf
Unsupervised hierarchical clustering revealed a strong similarity in gene modulation resulting from either compound treatment or BRAF...
Unsupervised hierarchical clustering revealed a strong similarity in gene modulation resulting from either compound treatment or BRAF ablation mediated by RNA interference relative to DMSO-treated control samples . Experiment Overall Design: We have generated melanoma A375 cells stably expressing a shRNA construct for doxycycline-inducible knockdown of B-Raf (2 mg/ml Dox, 48h). Small molecule treatment was at 1000nM R341787, 24h.
- Species:
- human
- Samples:
- 30
- Source:
- E-GEOD-13487
- PubMed:
- 19276360
- Updated:
- Dec.12, 2014
- Registered:
- Sep.11, 2014
Factors:
(via ArrayExpress)
| Sample | genotype | compound |
|---|---|---|
| GSE13487GSM339960 | wild type | RAF inhibitor #1 |
| GSE13487GSM339960 | wild type | RAF inhibitor #1 |
| GSE13487GSM339962 | sh2B-raf (doxycycline inducible) | RAF inhibitor #1 |
| GSE13487GSM339962 | sh2B-raf (doxycycline inducible) | RAF inhibitor #1 |
| GSE13487GSM339964 | shGFP | RAF inhibitor #2 |
| GSE13487GSM339965 | shB-Raf (doxycycline inducible) | RAF inhibitor #1 |
| GSE13487GSM339966 | wild type | BAY-43-9006 |
| GSE13487GSM339966 | wild type | BAY-43-9006 |
| GSE13487GSM339968 | sh2B-raf (doxycycline inducible) | BAY-43-9006 |
| GSE13487GSM339968 | sh2B-raf (doxycycline inducible) | BAY-43-9006 |
| GSE13487GSM339970 | shGFP | none |
| GSE13487GSM339971 | shB-Raf (doxycycline inducible) | none |
| GSE13487GSM339972 | sh2B-raf (doxycycline inducible) | none |
| GSE13487GSM339970 | shGFP | none |
| GSE13487GSM339971 | shB-Raf (doxycycline inducible) | none |
| GSE13487GSM339972 | sh2B-raf (doxycycline inducible) | none |
| GSE13487GSM339970 | shGFP | none |
| GSE13487GSM339971 | shB-Raf (doxycycline inducible) | none |
| GSE13487GSM339978 | wild type | DMSO |
| GSE13487GSM339979 | sh2B-raf (doxycycline inducible) | DMSO |
| GSE13487GSM339978 | wild type | DMSO |
| GSE13487GSM339979 | sh2B-raf (doxycycline inducible) | DMSO |
| GSE13487GSM339982 | shGFP | doxycycline |
| GSE13487GSM339983 | shB-Raf (doxycycline inducible) | doxycycline |
| GSE13487GSM339984 | sh2B-raf (doxycycline inducible) | doxycycline |
| GSE13487GSM339982 | shGFP | doxycycline |
| GSE13487GSM339983 | shB-Raf (doxycycline inducible) | doxycycline |
| GSE13487GSM339984 | sh2B-raf (doxycycline inducible) | doxycycline |
| GSE13487GSM339982 | shGFP | doxycycline |
| GSE13487GSM339983 | shB-Raf (doxycycline inducible) | doxycycline |