{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 1475, "factors": [{"GSM338408 1": {}}, {"GSM338407 1": {}}, {"GSM338406 1": {}}, {"GSM338405 1": {}}, {"GSM338404 1": {}}, {"GSM338403 1": {}}, {"GSM338402 1": {}}, {"GSM338401 1": {}}], "ownerprofile_id": "arrayexpress_sid", "platform": 3, "summary_wrapped": "Enhanced secondary Ab responses are a vital component of adaptive immunity, yet little is understood about the intrinsic and extrinsic...", "pubmed_id": 19124732, "geo_gse_id": "E-GEOD-13411", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 8, "tags": ["cell", "surface"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "gene-expression-by-human-splenic-b-cell-subsets", "geo_id_plat": "E-GEOD-13411_A-AFFY-33", "name": "Gene expression by human splenic B-cell subsets", "created": "Jun.19, 2014", "summary": "Enhanced secondary Ab responses are a vital component of adaptive immunity, yet little is understood about the intrinsic and extrinsic regulators of na\u00efve and memory B cells that results in differences in their responses to Ag. Microarray analysis, together with surface and intracellular phenotyping, revealed that memory B cells have increased expression of members of the TNF receptor, SLAM, B7 and Bcl2 families, as well as the TLR-related molecule CD180 (RP105). Accordingly, memory B cells exhibited enhanced survival, proliferation and Ig secretion, as well as entered division more rapidly than na\u00efve B cells in response to both T-dependent and T-independent stimuli. Furthermore, both IgM and isotype switched memory B cells, but not na\u00efve B cells, co-stimulated CD4+ T cells in vitro through a mechanism dependent on their constitutive expression of CD80 and CD86. This study demonstrates that upregulation of genes involved in activation, co-stimulation and survival provides memory B cells with a unique ability to produce enhanced immune responses and contributes to the maintenance of the memory B cell pool.  Keywords: cell type comparison Four subsets of human splenic B cells (na\u00efve, IgM-memory, Ig isotype switched memory and plasma cells); sort-purified and analysed immediately ex vivo; performed in duplicate.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13411", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13411/samples/"}