ArrayExpress Uploader03062non-targeting siRNAnon-targeting siRNAnon-targeting siRNAnon-targeting siRNAnon-targeting siRNAnon-targeting siRNABCL11A siRNABCL11A siRNABCL11A siRNABCL11A siRNABCL11A siRNABCL11A siRNAarrayexpress_sid4Differences in the amount of fetal hemoglobin (HbF) that persists into adulthood affect the severity of sickle cell disease and the beta-...19056937/profile/8773/arrayexpressuploader112celldiseasegene clusterhemoglobinthalassemiaDec.12, 2014FalseE-GEOD-13284_A-AFFY-44rnai-profiling-by-array-of-human-cd34-derived-erythttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13284RNAi profiling by array of human CD34-derived erythroid progenitors treated with BCL11A siRNAsSep.10, 2014Differences in the amount of fetal hemoglobin (HbF) that persists into adulthood affect the severity of sickle cell disease and the beta-thalassemia syndromes. Genetic association studies have identified sequence variants in the gene BCL11A that influence HbF levels. Here we examine BCL11A as a potential regulator of HbF expression. The high HbF BCL11A genotype is associated with reduced BCL11A expression. Moreover, abundant expression of full-length forms of BCL11A is developmentally restricted to adult erythroid cells. Down-regulation of BCL11A expression in primary adult erythroid cells leads to robust HbF expression. Consistent with a direct role of BCL11A in globin gene regulation, we find that BCL11A occupies several discrete sites in the beta-globin gene cluster. BCL11A emerges as a therapeutic target for reactivation of HbF in beta-hemoglobin disorders. BCL11A siRNA label: B, NT siRNA label: N Experiment Overall Design: Microarray expression analysis from CD34-derived erythroid progenitors treated with either non-targeting (NT) control siRNAs or BCL11A targeting siRNAs. Six samples from the NT control and six samples from the BCL11A siRNA treatment are included. Cells were harvested on day 7 of erythroid differentiation after introduction of siRNAs on day 0 of the differentiation protocol. Experiment Overall Design: 6 BCL11A siRNA datasets, 6 control (NT) datasetsE-GEOD-13284humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13284/samples/