{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 3062, "factors": [{"GSE13284GSM335322": {"RNA interference": "non-targeting siRNA"}}, {"GSE13284GSM335322": {"RNA interference": "non-targeting siRNA"}}, {"GSE13284GSM335322": {"RNA interference": "non-targeting siRNA"}}, {"GSE13284GSM335322": {"RNA interference": "non-targeting siRNA"}}, {"GSE13284GSM335322": {"RNA interference": "non-targeting siRNA"}}, {"GSE13284GSM335322": {"RNA interference": "non-targeting siRNA"}}, {"GSE13284GSM335328": {"RNA interference": "BCL11A siRNA"}}, {"GSE13284GSM335328": {"RNA interference": "BCL11A siRNA"}}, {"GSE13284GSM335328": {"RNA interference": "BCL11A siRNA"}}, {"GSE13284GSM335328": {"RNA interference": "BCL11A siRNA"}}, {"GSE13284GSM335328": {"RNA interference": "BCL11A siRNA"}}, {"GSE13284GSM335328": {"RNA interference": "BCL11A siRNA"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Differences in the amount of fetal hemoglobin (HbF) that persists into adulthood affect the severity of sickle cell disease and the beta-...", "pubmed_id": 19056937, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 12, "tags": ["cell", "disease", "gene cluster", "hemoglobin", "thalassemia"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-13284_A-AFFY-44", "slug": "rnai-profiling-by-array-of-human-cd34-derived-eryt", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13284", "geo_gds_id": "", "name": "RNAi profiling by array of human CD34-derived erythroid progenitors treated with BCL11A siRNAs", "created": "Sep.10, 2014", "summary": "Differences in the amount of fetal hemoglobin (HbF) that persists into adulthood affect the severity of sickle cell disease and the beta-thalassemia syndromes. Genetic association studies have identified sequence variants in the gene BCL11A that influence HbF levels. Here we examine BCL11A as a potential regulator of HbF expression. The high HbF BCL11A genotype is associated with reduced BCL11A expression. Moreover, abundant expression of full-length forms of BCL11A is developmentally restricted to adult erythroid cells. Down-regulation of BCL11A expression in primary adult erythroid cells leads to robust HbF expression. Consistent with a direct role of BCL11A in globin gene regulation, we find that BCL11A occupies several discrete sites in the beta-globin gene cluster. BCL11A emerges as a therapeutic target for reactivation of HbF in beta-hemoglobin disorders. BCL11A siRNA label: B, NT siRNA label: N Experiment Overall Design: Microarray expression analysis from CD34-derived erythroid progenitors treated with either non-targeting (NT) control siRNAs or BCL11A targeting siRNAs.  Six samples from the NT control and six samples from the BCL11A siRNA treatment are included.  Cells were harvested on day 7 of erythroid differentiation after introduction of siRNAs on day 0 of the differentiation protocol. Experiment Overall Design: 6 BCL11A siRNA datasets, 6 control (NT) datasets", "geo_gse_id": "E-GEOD-13284", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13284/samples/"}