{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSE13283GSM335312": {}}, {"GSE13283GSM335313": {}}, {"GSE13283GSM335314": {}}, {"GSE13283GSM335315": {}}, {"GSE13283GSM335316": {}}, {"GSE13283GSM335317": {}}, {"GSE13283GSM335318": {}}, {"GSE13283GSM335319": {}}, {"GSE13283GSM335320": {}}, {"GSE13283GSM335321": {}}], "id": 5527, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Differences in the amount of fetal hemoglobin (HbF) that persists into adulthood affect the severity of sickle cell disease and the beta-...", "geo_gse_id": "E-GEOD-13283", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 10, "tags": ["cell", "disease", "gene cluster", "hemoglobin", "thalassemia"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-13283_A-AFFY-45", "slug": "transcription-profiling-of-mouse-erythroleukemia-m", "geo_gds_id": "", "name": "Transcription profiling of mouse erythroleukemia (MEL) cells expressing tagged versions of BCL11A", "created": "Nov.10, 2014", "summary": "Differences in the amount of fetal hemoglobin (HbF) that persists into adulthood affect the severity of sickle cell disease and the beta-thalassemia syndromes. Genetic association studies have identified sequence variants in the gene BCL11A that influence HbF levels. Here we examine BCL11A as a potential regulator of HbF expression. The high HbF BCL11A genotype is associated with reduced BCL11A expression. Moreover, abundant expression of full-length forms of BCL11A is developmentally restricted to adult erythroid cells. Down-regulation of BCL11A expression in primary adult erythroid cells leads to robust HbF expression. Consistent with a direct role of BCL11A in globin gene regulation, we find that BCL11A occupies several discrete sites in the beta-globin gene cluster. BCL11A emerges as a therapeutic target for reactivation of HbF in beta-hemoglobin disorders. Expression clone label: FBB (4 different subclones, with 2 arrays each), Control label: MelBirA Experiment Overall Design: Microarray expression analysis from parental control mouse erythroleukemia (MEL) cells containing the BirA enzyme (MelBirA cells) and cells containing tagged versions (FLAG-Biotag) of BCL11A.  Two control datasets and eight datasets from four subclones containing tagged BCL11A are included.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13283", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13283/samples/"}