ArrayExpress Uploaderarrayexpress_sidhuman148303We addressed the clinical significance and mechanisms behind in vitro cellular responses to ionising radiation (IR)-induced DNA double...18941120E-GEOD-13280/profile/8773/arrayexpressuploader044Dec.12, 2014Falsetranscription-profiling-of-human-paediatric-acuteE-GEOD-13280_A-AFFY-33Transcription profiling of human paediatric acute lymphoblastic leukaemiaJun.19, 2014We addressed the clinical significance and mechanisms behind in vitro cellular responses to ionising radiation (IR)-induced DNA double strand breaks in 74 paediatric ALL patients. We found an apoptosis-resistant response in 36% of patients and an apoptosis-sensitive response in the remaining 64% of leukaemias. Global gene expression profiling of 11 apoptosis-resistant and 11 apoptosis-sensitive ALLs revealed abnormal up-regulation of multiple pro-survival pathways in response to IR in apoptosis-resistant leukaemias and differential post-transcriptional activation of the PI3-Akt pathway was observed in representative resistant cases. It is possible that abnormal pro-survival responses to DNA damage provide one of the mechanisms of primary resistance in ALL . Experiment Overall Design: Twenty two B-precursor ALL tumours (11 responsive to IR-induced DNA damage and 11 resistant) were analysed before and 8 hours after exposure to 5 Gy IR, using the Affymetrix HG_U133A (GPL96) platform.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13280http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13280/samples/