{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 3059, "factors": [{"GSM326147 1": {}}, {"GSM326148 1": {}}, {"GSM326149 1": {}}, {"GSM326150 1": {}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Goals and objectives of this study: to identify genes of the Treg signature induced by consitutive expression of GARP or FOXP3 in...", "pubmed_id": 19453521, "geo_gse_id": "E-GEOD-13234", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 4, "tags": ["cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "human-activated-treg-cells-and-retrovirally-engine", "geo_id_plat": "E-GEOD-13234_A-AFFY-44", "name": "Human activated Treg cells and retrovirally engineered Th cells, transduced with GARP, FOXP3 or control GFP vector", "created": "Sep.10, 2014", "summary": "Goals and objectives of this study: to identify genes of the Treg signature induced by consitutive expression of GARP or FOXP3 in antigen-specific Th cells with potential role for stabililization & maintenance of the regulatory program.  Keywords: T-cell receptor stimulation, gene-regulation, comparative gene expression profiling, cell type comparison, human, regulatory T cells, FOXP3, GARP Human alloantigen-specific Treg cells (THU) and Th cells (CD4-39), established and described recently (Ocklenburg et al.. Lab Invest.2006; 86: 724-737), were sitmulated for 3 days  with cognate antigen (EBV B cells) and IL2 as described  (Ocklenburg et al.. Lab Invest.2006; 86: 724-737) and analyzed using human Affymetrix U133 2.0 in monoplicate. Th cells had been transduced with a retroviral vector containing human GARP (LRRC32) or FOXP3 and an IRES-driven GFP as marker or empty GFP control, sorted for GFP+, expanded as described recently (Ocklenburg et al.. Lab Invest.2006; 86: 724-737), and characterized functially, phenotypically, and genetically as described (WO/2007/113301). Abbreviations: Treg THU d3 = alloantigen-specific Treg cells; GFP d3 = GFP-transduced alloantigen-specific Th cells (CD4-39); FoxP3 d3 = FOXP-transduced alloantigen-specific Th cells (CD4-39); Garp d3 = GARP-transduced alloantigen-specific Th cells (CD4-39); all stimulated for 3 days (=d3) with EBV B cells and IL2.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13234", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13234/samples/"}