ArrayExpress Uploaderarrayexpress_sidmouse551506The goal was to determine how IL-12 affects gene expression by murine CTL. Experiment Overall Design: Following RBC lysis, splenocytes...E-GEOD-13173/profile/8773/arrayexpressuploader04Dec.12, 2014FalseE-GEOD-13173_A-AFFY-45transcription-profiling-of-mouse-splenocytes-fromTranscription profiling of mouse splenocytes from OT-1 TCR transgenic mice to assess the effect of IL-12 on CTL gene expressionNov.10, 2014The goal was to determine how IL-12 affects gene expression by murine CTL. Experiment Overall Design: Following RBC lysis, splenocytes from OT-1 TCR transgenic mice (5 million/ml) were cultured in six well-plates (5-6ml/well) in IMDM supplemented with 10% FCS and 55μM 2-ME with 1μM SIINFEKL. After 4 days, the cells were harvested by purification with Ficoll-Paque PLUS and placed in fresh medium containing 0 or 20ng/ml rmIL-12. Twenty-four hours later the cells were harvested and used in experiments. The live cells harvested were >98% CD8+Vα2+ (OT-1).http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13173http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13173/samples/