{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSE13173GSM322634": {}}, {"GSE13173GSM322665": {}}, {"GSE13173GSM322667": {}}, {"GSE13173GSM322668": {}}], "id": 5515, "pop_total": 0, "platform": 6, "summary_wrapped": "The goal was to determine how IL-12 affects gene expression by murine CTL. Experiment Overall Design: Following RBC lysis, splenocytes...", "geo_gse_id": "E-GEOD-13173", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 4, "tags": [], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-13173_A-AFFY-45", "slug": "transcription-profiling-of-mouse-splenocytes-from", "geo_gds_id": "", "name": "Transcription profiling of mouse splenocytes from OT-1 TCR transgenic mice to assess the effect of IL-12 on CTL gene expression", "created": "Nov.10, 2014", "summary": "The goal was to determine how IL-12 affects gene expression by murine CTL. Experiment Overall Design: Following RBC lysis, splenocytes from OT-1 TCR transgenic mice (5 million/ml) were cultured in six well-plates (5-6ml/well) in IMDM supplemented with 10% FCS and 55\u03bcM 2-ME with 1\u03bcM SIINFEKL.  After 4 days, the cells were harvested by purification with Ficoll-Paque PLUS and placed in fresh medium containing 0 or 20ng/ml rmIL-12.  Twenty-four hours later the cells were harvested and used in experiments.  The live cells harvested were >98% CD8+V\u03b12+ (OT-1).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13173", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13173/samples/"}