ArrayExpress Uploader014340II3310Colon59sigma1800590390I2210Rectum54rectum lower third1800540510II3210Rectum47rectum lower third1300470750III3211Colon47colon descending-sigm.1101470541III33011Colon20caecum1502NA1840II3210Colon36colon transversum1500360670II3310Colon61sigma1400610610III3301Colon54colon ascending2601540741III3311Colon16sigma160131600I2200Rectum44rectum lower third2000440870II3310Colon53colon transversum1300530791IV3305Colon44caecum1412NANA700II3310Colon46sigma1300460760I2300Rectum50rectum2600500630I2210Rectum64rectum lower third2000640590III2201Rectum44rectum middle third2001440700III3201Colon50caecum2401500700I2200Colon52caecum2800520611III2318Rectum33rectum lower third2302NA1520I2310Rectum13rectum1300130760I2210Colon55colon transversum1300550670II3210Colon55sigma1400550650II3200Colon50colon transversum1600301591IV4316Colon21sigma2712NANA501IV33011Rectum25rectum middle to lower third2012NANA761IV32113Rectum23rectum upper third3812NANA601IV43020Colon10colon flexure right2512NANA570III3213Colon52sigma2201520440III4205Colon61sigma1902610580II3310Rectum52rectum middle third3000520730II3310Rectum37rectum lower third1400370660II3200Rectum54rectum lower third1300540600II3200Rectum54rectum lower third1300540600II3300Colon54colon descending1500540770II3310Rectum57rectum middle to upper third1600NA1660III3211Rectum58rectum upper third1401580490III3306Rectum7rectum lower third12x2NA0801II3200Rectum39rectum lower third1800361490I2200Rectum59rectum lower third1500590381III3314Rectum17rectum middle third2002101630II3210Colon59sigma1700590800III3301Rectum60rectum lower third1301600520I2310Colon43colon ascending2100430800II3200Rectum43rectum rektosigmoidaler transition3700430580I2310Rectum39rectum lower third1700390620II3210Colon43sigma3100430680II3210Rectum38rectum lower third4200380670III3305Rectum20rectum transition upper to middle third2802141820III3302Rectum37rectum lower third1201370590III3311Rectum33rectum middle third1601330420II3200Colon60sigma1400500390III3311Colon41sigma1201410640I2210Rectum49rectum lower third2300490710II3200Rectum64rectum lower third2300640650III3211Rectum35rectum lower third1201350810II3300Colon47sigma1200470790III4204Colon7colon ascending3302NANA680III2302Colon22colon ascending1201220670I2210Rectum51rectum lower third1500510631III3313Colon8colon2001NANA590II3310Rectum60rectum2100600651I2200Rectum32rectum middle to upper third130032080arrayexpress_sid3Wiskott-Aldrich syndrome (WAS) predisposes patients to leukemia and lymphoma. WAS is caused by mutations in the protein WASP which impair...19399471E-GEOD-12945/profile/8773/arrayexpressuploader1562breastbreast cancercancercolorectal cancerdiseasegliomaleukemialymphomaproteinsyndromewiskott-aldrich syndromeDec.12, 2014Falseexpression-data-from-colorectal-cancersE-GEOD-12945_A-AFFY-33Expression data from colorectal cancersJun.19, 2014Wiskott-Aldrich syndrome (WAS) predisposes patients to leukemia and lymphoma. WAS is caused by mutations in the protein WASP which impair its interaction with the WIPF1 protein. Here, we aim to identify a module of WIPF1-coexpressed genes and to assess its use as a prognostic signature for colorectal cancer, glioma, and breast cancer patients. Two public colorectal cancer microarray data sets were used for discovery and validation of the WIPF1 co-expression module. Based on expression of the WIPF1 signature, we classified more than 400 additional tumors with microarray data from our own experiments or from publicly available data sets according to their WIPF1 signature expression. This allowed us to separate patient populations for colorectal cancers, breast cancers, and gliomas for which clinical characteristics like survival times and times to relapse were analyzed. Groups of colorectal cancer, breast cancer, and glioma patients with low expression of the WIPF1 co-expression module generally had a favorable prognosis. In addition, the majority of WIPF1 signature genes are individually correlated with disease outcome in different studies. Literature gene network analysis revealed that among WIPF1 co-expressed genes known direct transcriptional targets of c-myc, ESR1 and p53 are enriched. The mean expression profile of WIPF1 signature genes is correlated with the profile of a proliferation signature. The WIPF1 signature is the first microarray-based prognostic expression signature primarily developed for colorectal cancer that is instrumental in other tumor types: low expression of the WIPF1 module is associated with better prognosis. We used microarrays for the validation of a WIPF1 co-expression module which was developed on two publically available datasets. Keywords: disease state analysis For the generation of our own microarray data set, 62 CRC patients undergoing elective standard oncological resection at the Department of General, Vascular and Thoracic Surgery, Campus Benjamin Franklin, Charité, were prospectively recruited.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12945humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12945/samples/