{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSE12802GSM321551": {}}, {"GSE12802GSM321552": {}}, {"GSE12802GSM321553": {}}, {"GSE12802GSM321554": {}}, {"GSE12802GSM321555": {}}, {"GSE12802GSM321556": {}}, {"GSE12802GSM321557": {}}, {"GSE12802GSM321558": {}}, {"GSE12802GSM321559": {}}, {"GSE12802GSM321560": {}}, {"GSE12802GSM321561": {}}, {"GSE12802GSM321562": {}}, {"GSE12802GSM321563": {}}, {"GSE12802GSM321564": {}}, {"GSE12802GSM321565": {}}, {"GSE12802GSM321566": {}}], "id": 5480, "pop_total": 0, "platform": 6, "summary_wrapped": "New insulin-producing pancreatic beta-cells are formed primarily by self-replication during adult life. To identify small molecules that...", "geo_gse_id": "E-GEOD-12802", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 16, "tags": ["beta cell", "cell", "insulin", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-12802_A-AFFY-45", "slug": "transcription-profiling-of-mouse-to-identify-small", "geo_gds_id": "", "name": "Transcription profiling of mouse to identify small molecules which induce pancreatic beta-cell expansion", "created": "Nov.10, 2014", "summary": "New insulin-producing pancreatic beta-cells are formed primarily by self-replication during adult life. To identify small molecules that can induce beta cell replication, a large chemical library was screened for proliferation of growth-arrested, reversibly immortalized mouse beta-cells using an automated high-throughput screening platform. A number of structurally diverse, active compounds were identified including phorbol esters, which likely act through protein kinase C, and a group of thiophene-pyrimidines that stimulate beta-cell proliferation by activating the Wnt signaling pathway. A group of dihydropyridine (DHP) derivatives was also shown to reversibly induce beta-cell replication in vitro by activating L-type calcium channels (LTCCs). Our data indicate that the LTCC agonist 2a affects the expression of genes involved in cell cycle progression and cellular proliferation. Furthermore, treatment of beta-cells with both LTCC agonist 2a and the Glp-1 receptor agonist Ex-4 showed an additive effect on beta-cell replication. The identification of small molecules that induce beta-cell proliferation suggests that it may be possible to reversibly expand other quiescent cells to overcome deficits associated with degenerative and/or autoimmune diseases. Experiment Overall Design: compound treatment and time course", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12802", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12802/samples/"}