ArrayExpress Uploaderarrayexpress_sidhumanempty vector controlempty vector controlempty vector controlempty vector controlempty vector controlempty vector controlHRas over expressionHRas over expressionHRas over expressionHRas over expressionHRas over expressionMEK1 over expressionMEK1 over expressionMEK1 over expressionMEK1 over expressionMEK1 over expressionMEK1 over expression301504MCF10A cells were then transfected with MEK1(S217S221), HRAS(G12V), and null control vectors; Cells were lysed 24 hours post-transfection.../profile/8773/arrayexpressuploader117proteinDec.12, 2014FalseE-GEOD-12764_A-AFFY-44transcription-profiling-by-array-of-human-mcf10a-2http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12764Transcription profiling by array of human MCF10A cells over-expressing HRas or MEK1Sep.10, 2014MCF10A cells were then transfected with MEK1(S217S221), HRAS(G12V), and null control vectors; Cells were lysed 24 hours post-transfection with collection of total RNA and protein Experiment Overall Design: Overexpression of Oncogenic protein was confirmed via Western blotE-GEOD-12764http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12764/samples/