4ArrayExpress Uploader0humannonenone nonenone nonenone tumor necrosis factor tumor growth factor 1tumor necrosis factor tumor growth factor 1tumor necrosis factor tumor growth factor 6tumor necrosis factor tumor growth factor 6tumor necrosis factor tumor growth factor 6tumor necrosis factor tumor growth factor 16tumor necrosis factor tumor growth factor 16tumor necrosis factor tumor growth factor 24tumor necrosis factor tumor growth factor 24tumor necrosis factor tumor growth factor 24tumor necrosis factor tumor growth factor 42tumor necrosis factor tumor growth factor 42tumor necrosis factor tumor growth factor 42tumor necrosis factor tumor growth factor 60tumor necrosis factor tumor growth factor 60tumor necrosis factor tumor growth factor 60tumor necrosis factor tumor growth factor 163005arrayexpress_sidhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12548The purpose of this study is to determine the changes in gene expression by a human retinal pigment epithelium (RPE) cell line (ARPE-19)...19965872/profile/8773/arrayexpressuploader320cellgenomelinepointDec.12, 2014Falsetranscription-profiling-by-array-of-a-human-retinaE-GEOD-12548_A-AFFY-44Transcription profiling by array of a human retinal pigment epithelium cell line after treatment with tumor necrosis factor and tumor growth factorSep.10, 2014The purpose of this study is to determine the changes in gene expression by a human retinal pigment epithelium (RPE) cell line (ARPE-19) in response to combination treatment of TGF and TNF, which induces phenotypic changes in vitro that mimic the EMT (Epithelial-to-Mesenchymal Transition). For this purpose, total RNA was extracted from TGF and TNF-treated ARPE-19 cells and differential gene expression between each time point (0, 1, 6, 16, 24, 42, and 60 hours) was determined using genechip arrays (Affymetrix, Human Genome U133). Experiment Overall Design: ARPE19 cell lines treated with TGF and TNF for 0, 1, 6, 16, 24, 42, and 60 hour. Each experiment were repeated three times. But 1 hour experiment was repeated two times.E-GEOD-12548http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12548/samples/