{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSE12367GSM310448": {}}, {"GSE12367GSM310449": {}}, {"GSE12367GSM310450": {}}, {"GSE12367GSM310452": {}}], "id": 5448, "pop_total": 0, "platform": 6, "summary_wrapped": "Microarray analysis was used to compare the gene expression profiles of Deaf-1-transduced mouse mammary epithelial cells (MECs) relative...", "geo_gse_id": "E-GEOD-12367", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 4, "tags": ["papilloma", "point"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-deaf-1-transduced", "geo_id_plat": "E-GEOD-12367_A-AFFY-45", "name": "Transcription profiling of mouse Deaf-1-transduced mammary epithelial cells", "created": "Nov.10, 2014", "summary": "Microarray analysis was used to compare the gene expression profiles of Deaf-1-transduced mouse mammary epithelial cells (MECs) relative to Deaf-1-deficient MECs. Experiment Overall Design: To investigate potential target genes of Deaf-1, primary mammary epithelial cells (MECs) were isolated from a Deaf-1-null mouse and immortalised by transduction with a retrovirus encoding the human papilloma virus (HPV16) E6/E7 proteins.  Two clones were selected and transduced with either an empty retrovirus or one encoding Deaf-1. RNA was subsequently isolated from cells at 24 and 48 hours after transduction for Affymetrix analysis.  High levels of Deaf-1 expression were demonstrated in cells transduced with the Deaf-1 retrovirus relative to controls following a 48 hour selection in puromycin.  This time-point was therefore selected for further analysis. Experiment Overall Design: To compare the gene expression profiles of Deaf-1-transduced MECs relative to Deaf-1-deficient MECs, Affymetrix analysis was performed using the GeneChip\u00ae Mouse Expression Set 430 2.0 array which comprises 39,000 transcripts on a single array.  Total RNA was harvested from two independent clones (C1 and E1) infected with either control or Deaf-1-expressing retrovirus making 4 samples in total.  Clones C1 and E1 were treated as biological replicates in the subsequent analysis.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12367", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12367/samples/"}