ArrayExpress Uploader08366arrayexpress_sid8The chromatin regulator Aiolos and the transcriptional coactivator OBF-1 have been implicated in regulating aspects of B cell maturation...18974788E-GEOD-12356/profile/8773/arrayexpressuploader08cellchromatincompartmentimmunoglobulinDec.12, 2014Falsetranscription-profiling-of-mouse-bone-marrow-withE-GEOD-12356_A-AFFY-36Transcription profiling of mouse bone marrow with Aiolos and OBF-1 deletions from 7 week old miceNov.24, 2014The chromatin regulator Aiolos and the transcriptional coactivator OBF-1 have been implicated in regulating aspects of B cell maturation and activation. Mice lacking either of these factors have a largely normal early B cell development. However, when both factors are eliminated simultaneously a block is uncovered at the transition between pre-B and immature B cells, indicating that these proteins exert a critical function in developing B lymphocytes. In mice deficient for Aiolos and OBF-1, the numbers of immature B cells are reduced, small pre-BII cells are increased and a significant impairment in immunoglobulin light chain DNA rearrangement is observed. We identified genes whose expression is deregulated in the pre-B cell compartment of these mice. In particular, we found that components of the pre-BCR, such as the surrogate light chain genes l5l5 and VpreB, fail to be efficiently silenced in double-mutant mice. Strikingly, developmentally regulated nuclear repositioning of the l5l5 gene is impaired in pre-B cells lacking OBF-1 and Aiolos. These studies uncover a novel role for OBF-1 and Aiolos in controlling the transcription and nuclear organization of genes involved in pre-BCR function. OBF-1 or Aiolos or both were deleted and the gene expression profiles for these animals investigated using Affymetrix arrays Experiment Overall Design: Two control wildtype animals, then duplicates for each of the single Aiolos or OBF-1 mutants and further duplicates for the Aiolos/OBF-1 double mutantshttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12356mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12356/samples/