ArrayExpress Uploader0humanextracted from xenografted 2MLN-GFP tumors.extracted from xenografted 2MLN-dnTbRII tumors.2992arrayexpress_sid4Diffuse-type gastric carcinoma is a poor-prognostic cancer with high expression of transforming growth factor (TGF)-β and thick stromal...E-GEOD-12336/profile/8773/arrayexpressuploader12cancercarcinomacellgastric carcinomaDec.12, 2014Falsegene-expression-analysis-of-tgf-beta-signaling-disE-GEOD-12336_A-AFFY-44Gene expression analysis of TGF-beta signaling-disrupting gastric cancer cell line 2MLNSep.10, 2014Diffuse-type gastric carcinoma is a poor-prognostic cancer with high expression of transforming growth factor (TGF)-β and thick stromal fibrosis. However, detailed investigations on the roles of TGF-β signaling in diffuse-type gastric carcinoma have not been performed. We generated two diffuse-type gastric carcinoma cell lines, dominant-negative TGF-β type II receptor expressing cells (2MLN-dnTβRII) and GFP-expressing cells (2MLN-GFP). Cells were subcutaneously or orthotopically injected into nude mice. Although dnTβRII did not affect the growth of OCUM-2MLN in vitro, it accelerated the growth of subcutaneously or orthotopically transplanted tumors in vivo. By microarray analysis, we found gene expression of TSP-1, an angiogenic inhibitor, was down-regulated in dnTβRII tumors. This results suggested disruption of TGF-β signaling in diffuse-type gastric carcinoma cells leads to alteration of tumor microenvironment and acceleration of tumor growth. We generated two OCUM-2MLN-derived gastric carcinoma cell lines; 2MLN-dnTβRII, which expresses dominant negative form of TβRII, and control 2MLN-GFP, which expresses GFP. The transplanted tumors of these cell lines were subjected to gene expression analysis with Affymetrix U133 plus2 arrays.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12336http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12336/samples/