{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM309953": {"TOTAL RNA": "extracted from xenografted 2MLN-GFP tumors."}}, {"GSM309954": {"TOTAL RNA": "extracted from xenografted 2MLN-dnTbRII tumors."}}], "id": 2992, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Diffuse-type gastric carcinoma is a poor-prognostic cancer with high expression of transforming growth factor (TGF)-\u03b2 and thick stromal...", "geo_gse_id": "E-GEOD-12336", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 2, "tags": ["cancer", "carcinoma", "cell", "gastric carcinoma"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "gene-expression-analysis-of-tgf-beta-signaling-dis", "geo_id_plat": "E-GEOD-12336_A-AFFY-44", "name": "Gene expression analysis of TGF-beta signaling-disrupting gastric cancer cell line 2MLN", "created": "Sep.10, 2014", "summary": "Diffuse-type gastric carcinoma is a poor-prognostic cancer with high expression of transforming growth factor (TGF)-\u03b2 and thick stromal fibrosis. However, detailed investigations on the roles of TGF-\u03b2 signaling in diffuse-type gastric carcinoma have not been performed. We generated two diffuse-type gastric carcinoma cell lines, dominant-negative TGF-\u03b2 type II receptor expressing cells (2MLN-dnT\u03b2RII) and GFP-expressing cells (2MLN-GFP). Cells were subcutaneously or orthotopically injected into nude mice. Although dnT\u03b2RII did not affect the growth of OCUM-2MLN in vitro, it accelerated the growth of subcutaneously or orthotopically transplanted tumors in vivo. By microarray analysis, we found gene expression of TSP-1, an angiogenic inhibitor, was down-regulated in dnT\u03b2RII tumors. This results suggested disruption of TGF-\u03b2 signaling in diffuse-type gastric carcinoma cells leads to alteration of tumor microenvironment and acceleration of tumor growth. We generated two OCUM-2MLN-derived gastric carcinoma cell lines; 2MLN-dnT\u03b2RII, which expresses dominant negative form of T\u03b2RII, and control 2MLN-GFP, which expresses GFP. The transplanted tumors of these cell lines were subjected to gene expression analysis with Affymetrix U133 plus2 arrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12336", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12336/samples/"}