ArrayExpress Uploader02990not specifiednot specifiednot specifiednot specifiednot specifiednot specifiedcontrol siRNAcontrolcontrol siRNAcontrolPOU5F1 siRNA-1EWS-POU5F1 knockdownPOU5F1 siRNA-1EWS-POU5F1 knockdownPOU5F1 siRNA-2EWS-POU5F1 knockdownPOU5F1 siRNA-2EWS-POU5F1 knockdownarrayexpress_sid4Our objective is to clarify the function of EWS-POU5F1 chimera. Specifially, GBS6 cells were established from an undifferentiated bone...20203285E-GEOD-12320/profile/8773/arrayexpressuploader29bonecelllinesarcomaDec.12, 2014Falsedifferential-gene-expression-in-gbs6-cells-after-eE-GEOD-12320_A-AFFY-44Differential gene expression in GBS6 cells after EWS-POU5F1 knockdownSep.10, 2014Our objective is to clarify the function of EWS-POU5F1 chimera. Specifially, GBS6 cells were established from an undifferentiated bone sarcoma carrying translocation t(6;22)(p21;q12). The translocation resulted in a gene fusion between EWS and POU5F1. Gene expression analysis of t(6;22) undifferentiated sarcoma cell line GBS6 transfected with POU5F1 specific siRNA to investigate the function of EWS-POU5F1. Knockdown of EWS-POU5F1 using POU5F1 specific siRNAs. 3 control and 6 experimental replicates representing the same experiment repeated 3 times (1st, 2nd, 3rd).http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12320humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12320/samples/