{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 2990, "factors": [{"GSM309615": {"TRANSFECTION": "not specified", "PROTOCOL": "not specified"}}, {"GSM309615": {"TRANSFECTION": "not specified", "PROTOCOL": "not specified"}}, {"GSM309615": {"TRANSFECTION": "not specified", "PROTOCOL": "not specified"}}, {"GSM468448": {"TRANSFECTION": "control siRNA", "PROTOCOL": "control"}}, {"GSM468448": {"TRANSFECTION": "control siRNA", "PROTOCOL": "control"}}, {"GSM468450": {"TRANSFECTION": "POU5F1 siRNA-1", "PROTOCOL": "EWS-POU5F1 knockdown"}}, {"GSM468450": {"TRANSFECTION": "POU5F1 siRNA-1", "PROTOCOL": "EWS-POU5F1 knockdown"}}, {"GSM468452": {"TRANSFECTION": "POU5F1 siRNA-2", "PROTOCOL": "EWS-POU5F1 knockdown"}}, {"GSM468452": {"TRANSFECTION": "POU5F1 siRNA-2", "PROTOCOL": "EWS-POU5F1 knockdown"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Our objective is to clarify the function of EWS-POU5F1 chimera. Specifially, GBS6 cells were established from an undifferentiated bone...", "pubmed_id": 20203285, "geo_gse_id": "E-GEOD-12320", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 9, "tags": ["bone", "cell", "line", "sarcoma"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "differential-gene-expression-in-gbs6-cells-after-e", "geo_id_plat": "E-GEOD-12320_A-AFFY-44", "name": "Differential gene expression in GBS6 cells after EWS-POU5F1 knockdown", "created": "Sep.10, 2014", "summary": "Our objective is to clarify the function of EWS-POU5F1 chimera. Specifially, GBS6 cells were established from an undifferentiated bone sarcoma carrying translocation t(6;22)(p21;q12). The translocation resulted in a gene fusion between EWS and POU5F1. Gene expression analysis of t(6;22) undifferentiated sarcoma cell line GBS6 transfected with POU5F1 specific siRNA to investigate the function of EWS-POU5F1. Knockdown of EWS-POU5F1 using POU5F1 specific siRNAs. 3 control and 6 experimental replicates representing the same experiment repeated 3 times (1st, 2nd, 3rd).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12320", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12320/samples/"}