{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 8365, "factors": [{"GSE12313GSM309523": {}}, {"GSE12313GSM309524": {}}, {"GSE12313GSM309525": {}}, {"GSE12313GSM309526": {}}], "ownerprofile_id": "arrayexpress_sid", "platform": 8, "summary_wrapped": "We created a mouse model where conditional expression of physiologic levels of an Mll-AF4 fusion oncogene induces development of acute...", "pubmed_id": 18977325, "geo_gse_id": "E-GEOD-12313", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 4, "tags": ["bone", "bone marrow"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-activated-or-cont", "geo_id_plat": "E-GEOD-12313_A-AFFY-36", "name": "Transcription profiling of mouse activated or control 5-FU bone marrow from MLL-AF4stop knockins", "created": "Nov.24, 2014", "summary": "We created a mouse model where conditional expression of physiologic levels of an Mll-AF4 fusion oncogene induces development of acute lymphoblastic (ALL) or acute myeloid leukemias (AML). Immunophenotypic and gene expression analysis of the ALL cells demonstrated bone marrow replacement with B-precursor cells which express a gene expression profile that has significant overlap with profiles in human MLL-rearranged ALL. To examine early, pre-leukemic changes in lymphoid cells due to Mll-AF4 expression, we infected 5-FU bone marrow cells from Mll-AF4stop knocking mice with activating (Cre-GFP) or control (MIF-GFP) retrovirus ex vivo and measured expression changes after culture under lymphoid growth conditions. Experiment Overall Design: Mll-AF4stop knock-in mice were treated with 5-FU and 5 days later their bone marrow infected ex vivo with either Cre-GFP to activate the Mll-AF4 fusion construct or with a control MIG-Cre retrovirus. GFP+ cells were sorted 2 days post-infection and cultured for 14 days under lymphoid growth conditions before total RNA was isolated for hybridization to Affymetrix expression microarrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12313", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12313/samples/"}