{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM309429": {"BIOSOURCEPROVIDER": "Lonza (Basel, Switzerland)", "PASSAGE": 4, "GROWTH MEDIUM": "Astrocyte Basal Medium (ABM\u2122) and the following growth supplements: rhEGF, 0.5 ml; Insulin, 1.25 ml; Ascorbic Acid, 0.5 ml; GA-1000, 0.5 ml, L-Glutamine, 5.0 ml; FBS, 15 ml."}}, {"GSM309429": {"BIOSOURCEPROVIDER": "Lonza (Basel, Switzerland)", "PASSAGE": 4, "GROWTH MEDIUM": "Astrocyte Basal Medium (ABM\u2122) and the following growth supplements: rhEGF, 0.5 ml; Insulin, 1.25 ml; Ascorbic Acid, 0.5 ml; GA-1000, 0.5 ml, L-Glutamine, 5.0 ml; FBS, 15 ml."}}, {"GSM309429": {"BIOSOURCEPROVIDER": "Lonza (Basel, Switzerland)", "PASSAGE": 4, "GROWTH MEDIUM": "Astrocyte Basal Medium (ABM\u2122) and the following growth supplements: rhEGF, 0.5 ml; Insulin, 1.25 ml; Ascorbic Acid, 0.5 ml; GA-1000, 0.5 ml, L-Glutamine, 5.0 ml; FBS, 15 ml."}}, {"GSM309433": {"BIOSOURCEPROVIDER": "Lonza (Basel, Switzerland)", "PASSAGE": 50, "GROWTH MEDIUM": "Dulbecco's Modified Eagle Medium and the following growth supplements: Penicillin/streptomycin, 5.0 ml; L-Glutamine, 5.0 ml; FBS, 15 ml."}}, {"GSM309435": {"BIOSOURCEPROVIDER": "ATCC", "PASSAGE": 50, "GROWTH MEDIUM": "Dulbecco's Modified Eagle Medium and the following growth supplements: Penicillin/streptomycin, 5.0 ml; L-Glutamine, 5.0 ml; FBS, 15 ml."}}, {"GSM309435": {"BIOSOURCEPROVIDER": "ATCC", "PASSAGE": 50, "GROWTH MEDIUM": "Dulbecco's Modified Eagle Medium and the following growth supplements: Penicillin/streptomycin, 5.0 ml; L-Glutamine, 5.0 ml; FBS, 15 ml."}}], "id": 2989, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Achievement of specific tumor cell targeting remains a challenge for glioma gene therapy. We report here the identification and...", "geo_gse_id": "E-GEOD-12305", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 6, "tags": ["brain", "cell", "glioma", "herpes simplex"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "a-5-sequence-of-human-hmgb2-gene-for-transcription", "geo_id_plat": "E-GEOD-12305_A-AFFY-44", "name": "A 5\u2019 Sequence of Human HMGB2 Gene for Transcriptional Targeting of Glioblastoma", "created": "Sep.10, 2014", "summary": "Achievement of specific tumor cell targeting remains a challenge for glioma gene therapy. We report here the identification and characterization of a 5\u2019 sequence of human HMGB2 gene for transcriptional targeting to glioblastoma. We performed microarray analysis and found HMGB2 as one of the genes that had a low level of expression in normal human astrocytes, but was significantly up-regulated in glioblastoma cells. Real-time PCR quantification revealed increase in HMBG2 expression level in glioblastoma tissues and cells between 11 to 79 fold over that in normal human brain tissue. With progressive truncation of a 5\u2019-upstream sequence of the HMGB2 gene, we identified a 500-bp fragment that displayed a high transcriptional activity in glioblastoma cells, but a low activity in normal brain cells. Using the sequence to drive the expression of the herpes simplex virus thymidine kinase gene in the context of a baculoviral vector, glioblastoma cells died in the presence of ganciclovir, whereas normal human astrocytes and neurons were not affected. We further confirmed that after intra-tumor injection, the baculoviral vector effectively suppressed the growth of human glioblastoma cells in a mouse xenograft model. Our results suggest that the 5\u2019-upstream sequence of the HMGB2 gene can be used as an efficient, tumor-selective promoter in targeted vectors for glioblastoma gene therapy.   U251 cells (n=3) genes level expression were compared to that of normal astrocytes (n=3) to find overexpressed genes in glioblastoma. Highly expressed genes were compared to those found in the litterature. This was selected to clone promoters of highly expressed genes in glioblastomas", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12305", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12305/samples/"}