ArrayExpress Uploader0rat9003arrayexpress_sid11An important component of time course microarray studies is the identification of genes that demonstrate significant time-dependent...E-GEOD-12289/profile/8773/arrayexpressuploader032glandpointFeb.09, 2015Falsetranscription-profiling-of-rat-samples-identifyingE-GEOD-12289_A-AFFY-43Transcription profiling of rat samples - identifying significant temporal variation in time series microarray data without replicatesJan.08, 2015An important component of time course microarray studies is the identification of genes that demonstrate significant time-dependent variation in their expression levels. Until recently available methods for performing such significance tests required replicates of individual time points. This paper describes a replicate-free method that was developed as part of a study of the estrous cycle in the rat mammary gland in which no replicate data was collected. Experiment Overall Design: Key points are examined in a time series over the four day estrus cycle of rat mammary gland in virgin (null parous). Singlet rats at each time point were used for mathematical analysis in this study for a total of 31 individual arrays.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12289http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12289/samples/