ArrayExpress Uploader02917Francisella tularensis novicidaFrancisella tularensis novicidaFrancisella tularensis novicidaFrancisella tularensis novicidaFrancisella tularensis Schu S4Francisella tularensis Schu S4Francisella tularensis Schu S4Francisella tularensis Schu S4uninfecteduninfecteduninfecteduninfecteduninfecteduninfectedarrayexpress_sid4Differential expression in human peripheral blood monocytes between F. novicida-infected and uninfected, and between Francisella...18698339E-GEOD-12108/profile/8773/arrayexpressuploader114leftperipheralDec.12, 2014Falsetranscription-profiling-by-array-of-human-periph-6E-GEOD-12108_A-AFFY-44Transcription profiling by array of human peripheral blood mononuclear cells infected with Francisella tularensisSep.03, 2014Differential expression in human peripheral blood monocytes between F. novicida-infected and uninfected, and between Francisella tularensis tularensis isolate Schu S4 and uninfected. The goal was to examine genomewide transcriptional reponses to these two strains, and identify differentially-regulated genes that may help explain the virulence of Schu S4. Experiment Overall Design: Human monocytes were infected with the Schu S4 isolate of Francisella tularensis tularensis (n=4), with F. tularensis subspecies novicida isolate U112 (n=4) or were left uninfected (n=6). Gene expression values were calculated using the gcrma package in R and BioConductor, and limma to identify differentially expressed genes. Submitted here are expression values calculated using R 2.7.1 and BioConductor 2.2 (FreeBSD/amd64) but the original were done using R 2.6.1 and BioConductor 2.1 (FreeBSD/amd64). Twelve other chips were pooled with these 14 for preprocessing.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12108humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-12108/samples/