ArrayExpress Uploader02909IGF-IR monoclonal antibody - 10H55IGF-IR monoclonal antibody - 10H55IGF-IR monoclonal antibody - 10H55IGF-IR monoclonal antibody - 10H55vehicle control vehicle control vehicle control vehicle control arrayexpress_sid4The insulin-like growth factor (IGF) system consists of two ligands (IGF-I and IGF-II), which both signal through type I IGF receptor...18790743E-GEOD-11959/profile/8773/arrayexpressuploader28breastbreast cancercancercellglucoseinsulinneuroblastomasurfaceDec.12, 2014Falsetranscription-profiling-by-array-of-human-sk-n-asE-GEOD-11959_A-AFFY-44Transcription profiling by array of human SK-N-AS neuroblastoma xenograft tumor treated with anti-human IGF-IR monoclonal antibody (h10H5) to study the anti-tumour function of the antibodySep.03, 2014The insulin-like growth factor (IGF) system consists of two ligands (IGF-I and IGF-II), which both signal through type I IGF receptor (IGF-IR) to stimulate proliferation and inhibit apoptosis, with activity contributing to malignant growth of many types of human cancers. We have developed a humanized, affinity-matured anti-human IGF-IR monoclonal antibody (h10H5), which binds with high affinity and specificity to the extracellular domain. h10H5 inhibits IGF-IR-mediated signaling by blocking IGF-I and IGF-IIbinding and by inducing cell surface receptor down-regulation via internalization and degradation. In vitro, h10H5 exhibits anti-proliferative effects on cancer cell lines. In vivo, h10H5 demonstrates single-agent anti-tumor efficacy in human SK-N-AS neuroblastoma and SW527 breast cancer xenograft models, and even greater efficacy in combination with the chemotherapeutic agent Docetaxel or an anti-VEGF antibody. Anti-tumor activity of h10H5 is associated with decreased AKT activation and glucose uptake, and a 316-gene transcription profile with significant changes involving DNA metabolic and cell cycle machineries. These data support the clinical testing of h10H5 as a biotherapeutic for IGF-IR-dependent human tumors. Experiment Overall Design: Two treatment groups with four tumor samples per group are collected and analyzed,http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-11959humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-11959/samples/