{"platform": 6, "owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSE11924GSM301396": {}}, {"GSE11924GSM301397": {}}], "id": 5433, "pop_total": 0, "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-11924", "summary_wrapped": "After activation, CD4+ helper T (Th) cells differentiate; into distinct effector subsets. Although chemokine; (C-X-C motif) receptor...", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 2, "tags": ["cell", "chemokine", "interleukin"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-11924_A-AFFY-45", "slug": "transcription-profiling-of-mouse-splenic-cd4cxcr5", "geo_gds_id": "", "name": "Transcription profiling of mouse Splenic CD4+CXCR5+ T (TFH) cells", "created": "Nov.10, 2014", "summary": "After activation, CD4+ helper T (Th) cells differentiate; into distinct effector subsets. Although chemokine; (C-X-C motif) receptor 5-expressing T follicular; helper (Tfh) cells are important in humoral immunity,; their developmental regulation is unclear. Here we; show that Tfh cells had a distinct gene expression; profile and developed in vivo independently of the; Th1 or Th2 cell lineages. Tfh cell generation was regulated; by ICOS ligand (ICOSL) expressed on B cells; and was dependent on interleukin-21 (IL-21), IL-6,; and signal transducer and activator of transcription; 3. However, unlike Th17 cells, differentiation of Tfh; cells did not require transforming growth factor; b (TGF-b) or Th17-specific orphan nuclear receptors; RORa and RORg in vivo. Finally, naive T cells activated; in vitro in the presence of IL-21 but not; TGF-b signaling preferentially acquired Tfh gene; expression and promoted germinal-center reactions; in vivo. This study thus demonstrates that Tfh is a; distinct Th cell lineage. Experiment Overall Design: Splenic CD4+CXCR5+ T cells were isolated from KLH-immunized mice and restimulated with anti-CD3 for 4 hours before total RNA preparation. Affymetrix gene chips were used to analyze their gene expression.", "geo_gse_id": "E-GEOD-11924", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-11924/samples/"}