ArrayExpress Uploader0mouse8356arrayexpress_sid8We analyzed whether cochlear removal-induced transcriptional changes in the cochlear nucleus (CN) were due to loss of electrical activity...E-GEOD-11726/profile/8773/arrayexpressuploader06cochleacochlear nucleusnerveDec.12, 2014FalseE-GEOD-11726_A-AFFY-36transcription-profiling-of-mouse-p21-cochlear-nuclTranscription profiling of mouse P21 cochlear nucleus after activity deprivationNov.24, 2014We analyzed whether cochlear removal-induced transcriptional changes in the cochlear nucleus (CN) were due to loss of electrical activity in the 8th nerve. Pharmacological activity blockade of the auditory nerve for 24 h resulted in similar expression changes for only a subset of genes. Thus, an additional factor not dependent on action potential-mediated signaling must also regulate transcriptional responses to deafferentation in the CN. Experiment Overall Design: TTX was infused into the cochlea unilaterally via osmotic pumps to block activity in the 8th nerve. CN tissue was dissected out from 10-12 mice per replicate and pooled. Three biological replicates were performed. Gene expression was compared between the ipsilateral and contralateral CN tissue to identify genes transcriptionally regulated after activity deprivation.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-11726http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-11726/samples/