{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM294970 1": {}}, {"GSM294971 1": {}}, {"GSM294972 1": {}}, {"GSM294973 1": {}}, {"GSM294974 1": {}}, {"GSM294975 1": {}}, {"GSM294976 1": {}}, {"GSM294977 1": {}}, {"GSM294978 1": {}}, {"GSM294979 1": {}}, {"GSM294980 1": {}}, {"GSM294981 1": {}}], "id": 5404, "pop_total": 0, "platform": 6, "summary_wrapped": "The molecular processes underlying the properties of ESC are yet unknown even when it's well established that LIF/STAT3 is neccesary for...", "geo_gse_id": "E-GEOD-11628", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 12, "tags": [], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "wnt-lif-signalling-in-mesc", "geo_id_plat": "E-GEOD-11628_A-AFFY-45", "name": "Wnt & LIF signalling in mESC", "created": "Nov.10, 2014", "summary": "The molecular processes underlying the properties of ESC are yet unknown even when it's well established that LIF/STAT3 is neccesary for the maintenance of pluripotency. Other pathways as Wnt are may be implicated in the regulation of the biological mechanisms in mESC. Work model: D3-ES cultivated with or without LIF and treated with chronic (7 days) low doses (50nM) of GSK3 \u03b2 inhibitor (lithium). We used microarrays to detail the global profile of gene expression to identified different groups of up and down-regulated genes in the work model. mESC cultivated as it has been defined in the work model were prepared for RNA extraction and hybridization on Affymetrix microarrays. We analyzed 3 independent experiments per replicate, and we did three replicates per sample.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-11628", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-11628/samples/"}