4ArrayExpress Uploader0human none none none10012estrogen10012estrogen10012estrogen1003estrogen1003estrogen1003estrogen1006estrogen1006estrogen1006estrogen2859arrayexpress_sidhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-11324The estrogen receptor is the master transcriptional regulator of breast cancer phenotype and the archetype of a molecular therapeutic...17013392/profile/8773/arrayexpressuploader312breastbreast cancercancerestrogengenomeDec.12, 2014Falsetranscription-profiling-by-array-of-human-mcf-cellE-GEOD-11324_A-AFFY-44Transcription profiling by array of human MCF cells treated with estrogenSep.03, 2014The estrogen receptor is the master transcriptional regulator of breast cancer phenotype and the archetype of a molecular therapeutic target. We mapped all estrogen receptor and RNA polymerase II binding sites on a genome-wide scale, identifying the authentic cis binding sites and target genes, in breast cancer cells. Combining this unique resource with gene expression data demonstrates distinct temporal mechanisms of estrogen-mediated gene regulation,particularly in the case of estrogen-suppressed genes. Furthermore, this resource has allowed the identification of cis-regulatory sites in previously unexplored regions of the genome and the cooperating transcription factors underlying estrogen signaling in breast cancer. Experiment Overall Design: This Series currently contains the gene expression data accompaning Carroll JS et al. Nature Genetics 38,1289-1297(2006). MCF7 cells were stimulated with 100 nM estrogen for 0, 3, 6, or 12h. All experiments were performed in triplicate.E-GEOD-11324http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-11324/samples/