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Home › Dataset Library › Transcription profiling of mouse BALB/c strain animals injected with breast cancer 4T1-derived cells to investigate the role of...

Dataset: Transcription profiling of mouse BALB/c strain animals injected with breast cancer 4T1-derived cells to investigate the role of epithelial to mesenchymal transition (EMT) in spontaneous breast cancer metastasis

Epithelial-mesenchymal transition (EMT) has been linked to cancer progression and metastatic propensity. The 4T1 tumor is a clinically...

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Epithelial-mesenchymal transition (EMT) has been linked to cancer progression and metastatic propensity. The 4T1 tumor is a clinically relevant model of spontaneous breast cancer metastasis. Here we characterize 4T1-derived cell lines for EMT, in vitro invasiveness and in vivo metastatic ability. Contrary to expectations, the 67NR cells, which form primary tumors but fail to metastasize, express vimentin and N-cadherin, but not E-cadherin. 4T1 cells, however, express E-cadherin, are highly migratory and invasive, and metastasize to multiple sites. The 66cl4 metastatic cells display mixed epithelial and mesenchymal markers, but are less migratory and invasive than 67NR cells. These findings demonstrate that the metastatic ability of breast cancer cells does not correlate with genotypic and phenotypic properties of EMT per se, and suggest that other processes may govern metastatic capability. Gene expression analysis also has not identified differences in EMT markers, but has identified several candidate genes that may influence metastatic ability. Experiment Overall Design: Female BALB/c mice were injected with 1x10^6 viable cells (3 mice with 4T1/CMVLUC, 3 mice with 66cl4/CMVLUC, and 3 mice with 67NR/CMVLUC) into the right fourth mammary gland. 15 days after injection primary tumors were excised, and total RNA for microarray hybridization was isolated from the tumor part of laser capture microdissected sections.

Species:
mouse

Samples:
9

Source:
E-GEOD-11259

PubMed:
18773493

Updated:
Dec.12, 2014

Registered:
Nov.10, 2014


Factors: (via ArrayExpress)
Sample CELLLINE INDIVIDUAL
GSE11259GSM284329 67NR 67NR_I
GSE11259GSM284330 67NR 67NR_II
GSE11259GSM284331 67NR 67NR_III
GSE11259GSM284332 66cl4 66cl4_I
GSE11259GSM284333 66cl4 66cl4_II
GSE11259GSM284334 66cl4 66cl4_III
GSE11259GSM284335 4T1 4T1_I
GSE11259GSM284336 4T1 4T1_II
GSE11259GSM284347 4T1 4T1_III

Tags

  • breast
  • breast cancer
  • cadherin
  • cancer
  • cell
  • e-cadherin
  • gland
  • n-cadherin
  • right
  • vimentin

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