{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSE11178GSM281723": {}}, {"GSE11178GSM281725": {}}, {"GSE11178GSM281722": {}}, {"GSE11178GSM281724": {}}], "id": 5364, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Ubiquitination is a post-translational mechanism of control of diverse cellular processes. We focus here on the ubiquitin ligase Fbw7, a...", "geo_gse_id": "E-GEOD-11178", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 4, "tags": ["bone", "bone marrow", "cell", "compartment", "genome", "hand", "thymus"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-fbw7-hematopoieti", "geo_id_plat": "E-GEOD-11178_A-AFFY-45", "name": "Transcription profiling of mouse Fbw7-/-/hematopoietic stem cells", "created": "Nov.10, 2014", "summary": "Ubiquitination is a post-translational mechanism of control of diverse cellular processes. We focus here on the ubiquitin ligase Fbw7, a recently identified hematopoietic tumor suppressor that can target for degradation several important oncogenes including Notch1, c-Myc and cyclin E. We have generated conditional Fbw7 knock-out animals and inactivated the gene in hematopoietic stem cells (HSC) and their differentiated progeny. Deletion of Fbw7 specifically and rapidly affects the HSC compartment in a cell-autonomous manner. Fbw7-/- HSCs show defective maintenance of quiescence, leading to impaired self-renewal and a severe loss of competitive repopulating capacity. Furthermore, Fbw7-/- HSC  are unable to colonize the thymus leading to a profound depletion of T cell progenitors. Deletion of Fbw7 in bone marrow stem cells and progenitors leads to the stabilization of c-Myc, a transcription factor previously implicated in HSC self-renewal. On the other hand, neither Notch1 nor cyclin E are stabilized in the bone marrow of Fbw7 deficient mice. Genome-wide transcriptome studies of Fbw7-/- HSC and hematopoietic progenitors indicate that Fbw7 controls, through the regulation of HSC cell cycle entry, the global transcriptional \u201csignature\u201d that is associated with the quiescent, self-renewing HSC phenotype. Transcriptional consequences of inactivating Fbw7 in LKS cells. Experiment Overall Design: Four samples were analyzed: wild-type (WT) control and Fbw7-deficient (FBW7) Lin-ckit+Sca1+ (LSK) cells, as well as Lin-ckit+Sca1- myeloid progenitor (MP) cells, which served as a control for LSK-enriched/specific genes.  Total bone marrow cells were pooled from three WT and three FBW7 mice before sorting LSK and MP populations.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-11178", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-11178/samples/"}