{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 8949, "factors": [{"GSE10770GSM273072": {}}, {"GSE10770GSM273072": {}}, {"GSE10770GSM273072": {}}, {"GSE10770GSM273072": {}}, {"GSE10770GSM273072": {}}, {"GSE10770GSM273072": {}}, {"GSE10770GSM273072": {}}, {"GSE10770GSM273072": {}}], "ownerprofile_id": "arrayexpress_sid", "platform": 13, "summary_wrapped": "Background; Mouse and rat models are mainstays in pharmacology, toxicology and drug development \u2013 but differences between strains and...", "pubmed_id": 18796159, "geo_gse_id": "E-GEOD-10770", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 8, "tags": ["class", "liver"], "lastmodified": "Jun.26, 2015", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-rat-samples-response-to", "geo_id_plat": "E-GEOD-10770_A-AFFY-25", "name": "Transcription profiling of rat samples response to dioxins (TCDD) to study the conserved transcriptional response of rat and mouse, see related set E-GEOD-10769 for mouse data", "created": "Jan.08, 2015", "summary": "Background; Mouse and rat models are mainstays in pharmacology, toxicology and drug development \u2013 but differences between strains and between species complicate data interpretation and application to human health. Dioxin-like polyhalogenated aromatic hydrocarbons represent a major class of environmentally and economically relevant toxicants. In mammals dioxin exposure leads to a broad spectrum of adverse affects, including hepatotoxicity of varying severity. Several studies have shown that dioxins extensively alter hepatic mRNA levels. Surprisingly, though, analysis of a limited portion of the transcriptome revealed that rat and mouse responses diverge greatly (Boverhof et al. Toxicol Sci 94:398\u2013416, 2006). Results; We employed oligonucleotide arrays to compare the response of 8,125 rat and mouse orthologs. We confirmed that there is limited inter-species overlap in dioxin-responsive genes. Rat-specific and mouse-specific genes are enriched for specific functional groups which differ between species, conceivably accounting for species-specificities in liver histopathology. While no evidence for the involvement of copy-number variation was found, extensive inter-species variation in the transcriptional-regulatory network was identified; Nr2f1 and Fos emerged as candidates to explain species-specific and species-independent responses, respectively. Conclusion; Our results suggest that a small core of genes is responsible for mediating the similar features of dioxin hepatotoxicity in rats and mice but non-overlapping pathways are simultaneously at play to result in distinctive histopathological outcomes. The extreme divergence between mouse and rat transcriptomic responses appears to reflect divergent transcriptional-regulatory networks. Taken together, these data suggest that both rat and mouse models should be used to screen the acute hepatotoxic effects of drugs and toxic compounds. Experiment Overall Design: Wild-type Long-Evans(Kuopio) rats were treated with 100 ug/kg TCDD (n=4) or with cornoil vehicle as control (n=4) for 19 hours. Their livers were then excised, RNA extracted, and the resulting samples hybridized to Affymetrix RAE230A arrays to survey changes in the transcriptome profile.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-10770", "species": "rat", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-10770/samples/"}