{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 5330, "factors": [{"GSM136067 1": {}}, {"GSM136068 1": {}}, {"GSM136069 1": {}}, {"GSM136070 1": {}}, {"GSM136071 1": {}}, {"GSM136072 1": {}}, {"GSM136073 1": {}}, {"GSM136074 1": {}}, {"GSM136075 1": {}}, {"GSM136076 1": {}}, {"GSM136077 1": {}}, {"GSM136078 1": {}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "[u'EXPERIMENT: Microarray expression profiles derived from the cranial neural folds (headfold) of neurulation-stage mouse embryos 3.0h...", "pubmed_id": 17200951, "geo_gse_id": "E-GEOD-1074", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 12, "tags": ["body", "cranial", "disease", "embryo", "fetal alcohol syndrome", "genome", "peripheral", "syndrome"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "strain-dependent-effects-of-alcohol-on-early-mouse", "geo_id_plat": "E-GEOD-1074_A-AFFY-45", "name": "Strain-dependent effects of alcohol on early mouse embryos", "created": "Nov.10, 2014", "summary": "[u'EXPERIMENT: Microarray expression profiles derived from the cranial neural folds (headfold) of neurulation-stage mouse embryos 3.0h after maternal alcohol exposure on 8 d.p.c. ANIMAL MODEL: Pregnancies from 2 substrains of C57BL/6 mice that differ in relative risk of Fetal Alcohol Syndrome (FAS): C57BL/6J (B6J) high-risk condition, and C57BL/6NCrl (B6N) low-risk condition. EXPOSURE: Dams dosed with ethanol (EtOH) by intraperitoneal injection at 2.9 g EtOH per kg body weight. Controls received vehicle (saline) alone. A third group of dams received EtOH + 4.0 mg/kg PK11195, a specific high-affinity ligand to the 18 KDa mitochondrial peripheral benzodiazepine receptor site. INTERVAL: High blood alcohol content must be sustained in dams for several hours to invoke FAS and is traditionally accomplished by a double injection of EtOH 4.0h apart. Since these embryos were harvested for genetic analysis 1h before dams would have gotten the second alcohol injection, the interval represents a snapshot of the critical response, prior to the second maternal injection that invokes greater risk. Counter-exposure to PK11195 significantly lowers the adverse response of B6J embryos to EtOH. PLATFORM: Two independent assays run. The first dataset (PE), run April 2002 \\u2013 January 2003, used samples pooled from 2 litters (PE) and the platform was a two-channel MPS621 array (', {u'a': {u'href': u'http://www.lifesciences.perkinelmer.com/', u'target': u'_blank', u'$': u'http://www.lifesciences.perkinelmer.com/'}}, u'). This platform has 4800 sequence-verified gene elements derived from over 50 different human cDNA libraries reflecting a variety of well-annotated cellular processes and disease pathways. The second dataset (AF), run between May 2005 \\u2013 November 2005, used samples pooled from 1 litter and the platform was Affymetrix GeneChip\\xae Mouse Genome 430A 2.0 Array (', {u'a': {u'href': u'http://www.affymetrix.com/', u'target': u'_blank', u'$': u'http://www.affymetrix.com/'}}, u'). The MG430A 2.0 platform has 45,102 oligonucleotide probe cells representing approximately 14,000 well-characterized genes in the draft mouse genome assembly. The corresponding GEO samples are GSM12218 - GSM12227 for the two-channel PE arrays, and GSM136067 - GSM136078 for the one-channel AF arrays. Keywords = fetal alcohol syndrome Keywords = alcohol-related birth defects Keywords = EtOH Keywords = PK11195 Keywords = embryo Keywords = strain differences Keywords: Ordered Assay of early mouse embryos during pathogenesis of Fetal Alcohol Syndrome (FAS). Replicate RNA samples were arrayed by one-channel oligonucleotide (Affymetrix) or two-channel cDNA (Perkin-Elmer) platforms.']", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-1074", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-1074/samples/"}