{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSE10627GSM267881": {}}, {"GSE10627GSM267882": {}}, {"GSE10627GSM267883": {}}, {"GSE10627GSM267884": {}}, {"GSE10627GSM267885": {}}, {"GSE10627GSM267886": {}}, {"GSE10627GSM267887": {}}, {"GSE10627GSM267873": {}}, {"GSE10627GSM267874": {}}, {"GSE10627GSM267875": {}}, {"GSE10627GSM267876": {}}, {"GSE10627GSM267877": {}}, {"GSE10627GSM267878": {}}, {"GSE10627GSM267879": {}}, {"GSE10627GSM267880": {}}, {"GSE10627GSM267869": {}}, {"GSE10627GSM267870": {}}, {"GSE10627GSM267871": {}}, {"GSE10627GSM267872": {}}, {"GSE10627GSM267888": {}}, {"GSE10627GSM267889": {}}, {"GSE10627GSM267890": {}}, {"GSE10627GSM267891": {}}, {"GSE10627GSM267892": {}}, {"GSE10627GSM267856": {}}, {"GSE10627GSM267857": {}}, {"GSE10627GSM267858": {}}, {"GSE10627GSM267859": {}}, {"GSE10627GSM267860": {}}, {"GSE10627GSM267861": {}}, {"GSE10627GSM267862": {}}, {"GSE10627GSM267863": {}}, {"GSE10627GSM267847": {}}, {"GSE10627GSM267848": {}}, {"GSE10627GSM267849": {}}, {"GSE10627GSM267850": {}}, {"GSE10627GSM267851": {}}, {"GSE10627GSM267852": {}}, {"GSE10627GSM267853": {}}, {"GSE10627GSM267854": {}}, {"GSE10627GSM267855": {}}, {"GSE10627GSM267842": {}}, {"GSE10627GSM267843": {}}, {"GSE10627GSM267844": {}}, {"GSE10627GSM267845": {}}, {"GSE10627GSM267846": {}}, {"GSE10627GSM267864": {}}, {"GSE10627GSM267865": {}}, {"GSE10627GSM267866": {}}, {"GSE10627GSM267867": {}}, {"GSE10627GSM267868": {}}], "id": 5320, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "The pathways by which oncogenes, such as MLL-AF9, initiate transformation and leukemia in  humans and mice are incompletely defined. In a...", "geo_gse_id": "E-GEOD-10627", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 51, "tags": ["cell", "leukemia", "monocyte"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-hematopoietic-cel", "geo_id_plat": "E-GEOD-10627_A-AFFY-45", "name": "Transcription profiling of mouse hematopoietic cells (GMP, CMP, CLP and HSC), FACS sorted from wild type and Mll-AF9 knock-ins", "created": "Nov.10, 2014", "summary": "The pathways by which oncogenes, such as MLL-AF9, initiate transformation and leukemia in  humans and mice are incompletely defined. In a study of target cells and oncogene dosage, we found that  Mll-AF9, when under endogenous regulatory control, efficiently transformed LSK (Lin- Sca1+ c-kit+) stem  cells while committed granulocyte-monocyte progenitors (GMPs) were transformation-resistant and did not  cause leukemia. Mll-AF9 was expressed at higher levels in hematopoietic stem (HSC) than GMP cells. Mll-  AF9 gene dosage effects were directly shown in experiments where GMPs were efficiently transformed by  the high dosage of Mll-AF9 resulting from retroviral transduction. Mll-AF9 up-regulated expression of 196  genes in both LSK and progenitor cells, but to higher levels in LSKs than in committed myeloid progenitors. Experiment Overall Design: Comparison of gene expression profiles among four types of hematopoietic cells (GMP, CMP, CLP and HSC), FACS sorted from wild type and Mll-AF9 knock-in mice. The goal was to identify genes differentially expressed in each Mll-AF9 cell type compared to the corresponding wild type cells.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-10627", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-10627/samples/"}