ArrayExpress Uploader0humanCD14-postive monocytesCD14-postive monocytesno CD14-positive monocytesno CD14-positive monocytesCD14-postive monocytesCD14-postive monocytesno CD14-positive monocytesno CD14-positive monocytes2749arrayexpress_sid4The hematopoietic microenvironment consists of non-hematopoietic derived stromal elements and hematopoietic derived monocytes and...E-GEOD-10595/profile/8773/arrayexpressuploader18bonebone marrowcelllinemonocytestromastromal cellDec.12, 2014Falsetranscription-profiling-by-array-of-human-bone-m-3E-GEOD-10595_A-AFFY-44Transcription profiling by array of human bone marrow stromal cells lines cultured with monocytes to investigate interactions in the hematopoietic microenvironmentAug.12, 2014The hematopoietic microenvironment consists of non-hematopoietic derived stromal elements and hematopoietic derived monocytes and macrophages which interact and function together to control the proliferation and differentiation of early blood-forming cells. Two human stromal cell lines (HS-5 and HS-27a) representing distinct functional components of this microenvironment have been extensively characterized and shown to influence monocyte gene expression. This series of gene expression profiles is intended to extend the previous studies and identify which gene expression changes may require cell-cell contact or occur in the stromal cells as a result of monocyte influence;or in the monocytes as a result of stormal influences. Experiment Overall Design: Two human bone marrow stromal cell lines (HS5 and HS27a) were cultured with and without monocytes (CD14+ cells) from 2 different donors. Experiment Overall Design: culture condition: stroma no monocytes: HS5-1, HS5-2, HS27a-1, HS27a-2 Experiment Overall Design: culture condition: stroma with monocytes: HS5-MO-1, HS5-MO-2, HS27a-MO-1, HS27a-MO-2 Experiment Overall Design: donor: donor 3: HS5-MO-1, HS27a-MO-1 Experiment Overall Design: donor: donor 4: HS5-MO-2, HS27a-MO-2 Experiment Overall Design: cell line: HS5: HS5-1, HS5-2, HS5-MO-1, HS5-MO-2 Experiment Overall Design: cell line: HS27a: HS27a-1, HS27a-2, HS27a-MO-1, HS27a-MO-2http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-10595http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-10595/samples/