{"platform": 6, "owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSE10422GSM263652": {}}, {"GSE10422GSM263653": {}}, {"GSE10422GSM263654": {}}, {"GSE10422GSM263643": {}}, {"GSE10422GSM263645": {}}, {"GSE10422GSM263646": {}}, {"GSE10422GSM263642": {}}, {"GSE10422GSM263640": {}}, {"GSE10422GSM263641": {}}, {"GSE10422GSM263647": {}}, {"GSE10422GSM263648": {}}, {"GSE10422GSM263644": {}}, {"GSE10422GSM263649": {}}, {"GSE10422GSM263650": {}}, {"GSE10422GSM263651": {}}], "id": 5300, "ownerprofile_id": "arrayexpress_sid", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-10422", "summary_wrapped": "Tumor necrosis factor-associated factors 2 and 3 (TRAF2 and TRAF3) were shown to function in a co-operative and non-redundant manner to...", "pubmed_id": 18313334, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 15, "tags": ["cell", "genome", "lymph", "lymph node"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-lymph-node-derive", "geo_id_plat": "E-GEOD-10422_A-AFFY-45", "name": "Transcription profiling of mouse lymph node derived B cells from Traf2 and Traf3 B cell knockout mice and Baff tg mice", "created": "Nov.10, 2014", "summary": "Tumor necrosis factor-associated factors 2 and 3 (TRAF2 and TRAF3) were shown to function in a co-operative and non-redundant manner to suppress nuclear factor-\u03baB2 (NF-\u03baB2) activation, gene expression and survival in mature B cells. In the absence of this suppressive activity, B cells developed independently of the obligatory B cell survival factor, BAFF (B cell activating factor of the tumor necrosis factor family).  This constitutive, lineage-specific suppression of B cell survival by TRAF2 and TRAF3 determines the requirement for BAFF to sustain B cell development in vivo.  We wished to investigate the effect on gene expression in B cells which lacked the negative regulators TRAF2 and TRAF3, and hence had hyperactive NF-kB2 signalling.  As Baff-tg mice display a similar phenotype, and have a genetic modification which acts in the same pathway, yet further up, than TRAF2 and TRAF3, we wished to compare and contrast Baff-tg B cells with TRAF2 and TRAF3 deficient B cells.  This analysis should identify genes that are important in B cell survival. Experiment Overall Design: Lymph node B cells were purified from Traf2 B cell knockout mice, Traf3 B cell knockout mice, Baff-tg mice and respective controls.  RNA was extracted and hybridised to Affymetrix 430 2.0 Mouse Genome Arrays.  Samples were processed and hence analysed on three spearate days.  Day 1 two control mice: Traf2lox/lox pool and CD19-cretg were compared to two knockout mice: Traf2DB 80 and Traf3DB 94.  On Day 2 three control mice: Traf2lox/lox 77, Traf2lox/lox 79 and Traf3lox/lox 97 were compared to two knockout mice: Traf2DB 76 and Traf3DB 01.  On Day 3 three control mice: WT33, WT34, WT35 were compared to three Baff-tg mice: Baff-tg 99, Baff-tg 100, Baff-tg 101.", "geo_gse_id": "E-GEOD-10422", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-10422/samples/"}