Dataset: Transcription profiling of mouse SMARTA transgenic cells in response to LCMV or Lm-gp61 infection

Following infection with LCMV, CD4+ SMARTA TCR transgenic cells (specific for the gp61-80 epitope of the LCMV glycoprotein) rapidly expand, become effector cells, and go on to form a long-lived memory population. Following infection with a recombinant Listeria monocytogenes expressing the LCMV epitope gp61-80, SMARTA cells also expand but display defective effector differentiation and fail to form memory. In an attempt to understand the signals required for CD4 T cell memory differentiation, we compared gene expression by SMARTA cells at the peak of the primary response following either Lm-gp61 or LCMV infection. Experiment Overall Design: SMARTA cells were purified at day 7 post-infection with either LCMV or Lm-gp61. SMARTA cells were sorted on the basis of Thy1.1 expression using a FACSAria. Cells were sorted through the machine twice to enhance purity. Two biological replicates of each group are provided. Each replicate represents the results of SMARTA pooled from three animals.

Species:

mouse

Samples:

4

Source:

E-GEOD-10094

Updated:

Dec.12, 2014

Registered:

Nov.10, 2014