ArrayExpress Uploaderarrayexpress_sid8219TCDD 10µg/kg i.p. injectedDMSO i.p. injectedTCDD 10µg/kg i.p. injectedDMSO i.p. injected078-12 week, female C57BL/6 mice were injected with 10 µg/kg TCDD or solvent control. CD8+ T cells from spleen were FACS purified and...16972790E-GEOD-10093/profile/8773/arrayexpressuploader14cellperipheralspleenDec.12, 2014FalseE-GEOD-10093_A-AFFY-23effect-of-tcdd-exposure-on-purified-splenic-cd8-tEffect of TCDD Exposure on purified splenic CD8 T cellsNov.23, 20148-12 week, female C57BL/6 mice were injected with 10 µg/kg TCDD or solvent control. CD8+ T cells from spleen were FACS purified and submitted to transcription profiling Keywords: TCDD, AHR, Immunosupression peripheral T cells, CD8 T cells, Toxicogenomics For each group 4 female C57BL/6 mice were treated, spleen surgically removed and single cell suspensions generated. Cell suspensions were pooled, and CD8 positive T cells purified (purity > 99%). RNA was isolated using TRIzol and amplified using the Ambion message AMP kit. Target lableing and hybridization was performed using Affymetrix standard protocolshttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-10093mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-10093/samples/