{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "id": 8219, "factors": [{"GSM254976": {"TREATMENT": "TCDD 10\u00b5g/kg i.p. injected"}}, {"GSM254977": {"TREATMENT": "DMSO i.p. injected"}}, {"GSM254976": {"TREATMENT": "TCDD 10\u00b5g/kg i.p. injected"}}, {"GSM254977": {"TREATMENT": "DMSO i.p. injected"}}], "pop_total": 0, "platform": 7, "summary_wrapped": "8-12 week, female C57BL/6 mice were injected with 10 \u00b5g/kg TCDD or solvent control. CD8+ T cells from spleen were FACS purified and...", "pubmed_id": 16972790, "geo_gse_id": "E-GEOD-10093", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 4, "tags": ["cell", "peripheral", "spleen"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-10093_A-AFFY-23", "slug": "effect-of-tcdd-exposure-on-purified-splenic-cd8-t", "geo_gds_id": "", "name": "Effect of TCDD Exposure on purified splenic CD8 T cells", "created": "Nov.23, 2014", "summary": "8-12 week, female C57BL/6 mice were injected with 10 \u00b5g/kg TCDD or solvent control. CD8+ T cells from spleen were FACS purified and submitted to transcription profiling Keywords: TCDD, AHR, Immunosupression peripheral T cells, CD8 T cells, Toxicogenomics For each group 4 female C57BL/6 mice were treated, spleen surgically removed and single cell suspensions generated. Cell suspensions were pooled, and CD8 positive T cells purified (purity > 99%). RNA was isolated using TRIzol and amplified using the Ambion message AMP kit. Target lableing and hybridization was performed using Affymetrix standard protocols", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-10093", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-10093/samples/"}