{"platform": 4, "owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSE10070GSM254523": {"phenotype": "monolayer control"}}, {"GSE10070GSM254523": {"phenotype": "monolayer control"}}, {"GSE10070GSM254523": {"phenotype": "monolayer control"}}, {"GSE10070GSM254538": {"phenotype": "low transepithelial electrical resistance"}}, {"GSE10070GSM254538": {"phenotype": "low transepithelial electrical resistance"}}, {"GSE10070GSM254538": {"phenotype": "low transepithelial electrical resistance"}}, {"GSE10070GSM254615": {"phenotype": "mid transepithelial electrical resistance"}}, {"GSE10070GSM254615": {"phenotype": "mid transepithelial electrical resistance"}}, {"GSE10070GSM254615": {"phenotype": "mid transepithelial electrical resistance"}}, {"GSE10070GSM254618": {"phenotype": "plateau transepithelial electrical resistance"}}, {"GSE10070GSM254618": {"phenotype": "plateau transepithelial electrical resistance"}}, {"GSE10070GSM254618": {"phenotype": "plateau transepithelial electrical resistance"}}, {"GSE10070GSM254618": {"phenotype": "plateau transepithelial electrical resistance"}}], "id": 2722, "ownerprofile_id": "arrayexpress_sid", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-10070", "summary_wrapped": "To further understand the differences occurring in MCF10A cells as they polarize and differentiate in the Transwell\u00ae model, we performed...", "pubmed_id": 19005683, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 13, "tags": ["genome", "point"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "gene-expression-in-mcf10a-cells-through-differenti", "geo_id_plat": "E-GEOD-10070_A-AFFY-44", "name": "Gene Expression in MCF10A cells through Differentiation on Transwells", "created": "Aug.12, 2014", "summary": "To further understand the differences occurring in MCF10A cells as they polarize and differentiate in the Transwell\u00ae model, we performed gene expression profiling with Affymetrix Human Genome U133 Plus 2.0 Arrays.  Four experimental time points, were sampled:  conventional cultures of MCF10A cells grown on plastic (Monolayer) and MCF10A cells plated on Transwells\u00ae sampled at three TEER values, 200-300 \u03a9 cm2 (Base), 1400-1600 \u03a9 cm2 (Midpoint), and 3000-3200 \u03a9 cm2 (Plateau).  Experiment Overall Design: Cells are grown in monolayer to 90-95% confluency, trypsinized and counted for seeding onto permeable supports (Transwell\u00ae, 0.4 \u00b5m pores, polyester) in normal growth medium.  MCF10A cells are seeded on 12-well Transwells\u00ae (Corning) at 105 cells/cm2.  Both chambers of media were changed strictly on a 24-hour schedule.  Transepithelial electrical resistance (TEER) is measured daily with Epithelial Volt-Ohm Meter (EVOM; World Precision Instruments), prior to media change.  Total RNA was isolated at the indicated TEER listed above.  Canonical monolayer MCF10A cells served as a control comparison.  Each time point was performed on triplicate arrays except for Plateau (n=4).", "geo_gse_id": "E-GEOD-10070", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-10070/samples/"}