{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 5263, "factors": [{"GSE10011GSM252936": {}}, {"GSE10011GSM252937": {}}, {"GSE10011GSM252938": {}}, {"GSE10011GSM252939": {}}, {"GSE10011GSM252940": {}}, {"GSE10011GSM252941": {}}, {"GSE10011GSM252942": {}}, {"GSE10011GSM252943": {}}, {"GSE10011GSM252944": {}}, {"GSE10011GSM252945": {}}, {"GSE10011GSM252946": {}}, {"GSE10011GSM252947": {}}, {"GSE10011GSM252948": {}}, {"GSE10011GSM252949": {}}, {"GSE10011GSM252950": {}}, {"GSE10011GSM252951": {}}, {"GSE10011GSM252952": {}}, {"GSE10011GSM252953": {}}, {"GSE10011GSM252954": {}}, {"GSE10011GSM252955": {}}, {"GSE10011GSM252956": {}}, {"GSE10011GSM252957": {}}, {"GSE10011GSM252958": {}}, {"GSE10011GSM252959": {}}, {"GSE10011GSM252960": {}}, {"GSE10011GSM252961": {}}, {"GSE10011GSM252962": {}}, {"GSE10011GSM252963": {}}, {"GSE10011GSM252964": {}}, {"GSE10011GSM252965": {}}, {"GSE10011GSM252966": {}}, {"GSE10011GSM252967": {}}, {"GSE10011GSM252968": {}}, {"GSE10011GSM252969": {}}, {"GSE10011GSM252970": {}}, {"GSE10011GSM252971": {}}, {"GSE10011GSM252972": {}}, {"GSE10011GSM252973": {}}, {"GSE10011GSM252974": {}}, {"GSE10011GSM252975": {}}, {"GSE10011GSM252976": {}}, {"GSE10011GSM252977": {}}, {"GSE10011GSM252978": {}}, {"GSE10011GSM252979": {}}, {"GSE10011GSM252980": {}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Data from tc-, nt- and p-RNA as well as 1 and 2h of actinomycin-D treatment (5\u00b5g/ml) of NIH-3T3 cells used to determine half-lives. RNA...", "pubmed_id": 21385826, "geo_gse_id": "E-GEOD-10011", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 45, "tags": ["cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-nih-3t3-cells-for", "geo_id_plat": "E-GEOD-10011_A-AFFY-45", "name": "Transcription profiling of mouse NIH-3T3 cells for tc-, nt- and p-RNA half-life determination", "created": "Nov.10, 2014", "summary": "Data from tc-, nt- and p-RNA as well as 1 and 2h of actinomycin-D treatment (5\u00b5g/ml) of NIH-3T3 cells used to determine half-lives. RNA was labeled for 15, 30 or 60 minutes with 4-thiouridine. After preparation of tc-RNA, thiol-labeled RNA was biotinylated using biot-HPDP and subsequently tc-RNA was separated into nt- and p-RNA using streptavidin coated magnetic beads. All three fractions were used for microarray analysis. For actinomycin-D experiments only tc-RNA was used prepared from cell before and 1 an 2h after addition of act-D. We used microarrays to analyze the effects of 1 and 3h of IFNalpha and gamma treatment in total cellular RNA Experiment Overall Design: NIH-3T3 cells ( 5th to 15th passage after thawing) were split from confluent plates 24h before start of the experiment. At the begin of the experiment about 80% confluency was reached. The experiment was started by applying fresh, prewarmed, CO2-equilibrated medium containing either 200\u00b5M 4sU (for 1h labeling), 500\u00b5M 4sU (for <=30min labeling) or 5\u00b5g/ml actinomycin-D.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-10011", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-10011/samples/"}