ArrayExpress Uploaderarrayexpress_sid88841,000,0000.0241,000,0000.0241,000,0000.0241,000,0000.0241,000,0000.0241,000,0000.0241,000,0000.0241,000,0000.0241,000,0000.061,000,0000.061,000,0000.061,000,0000.061,000,0000.061,000,0000.061,000,0000.061,000,0000.061,000,0000.1241,000,0000.1241,000,0000.1241,000,0000.1241,000,0000.1241,000,0000.1241,000,0000.1241,000,0000.1241,000,0000.161,000,0000.161,000,0000.161,000,0000.161,000,0000.161,000,0000.1600.12400.12400.12400.12400.12400.12400.12400.12400.1600.1600.1600.1600.1600.1600.1600.16 0.024 0.024 0.024 0.024 0.024 0.024 0.024 0.024 0.06 0.06 0.06 0.06 0.06 0.06 0.06 0.0609Viral infections and local production of IFNgamma might contribute to beta-cell dysfunction/death in Type 1 diabetes. Double stranded RNA...14600816E-CBIL-11/profile/8773/arrayexpressuploader362cellFeb.09, 2015FalseE-CBIL-11_A-AFFY-18transcription-profiling-of-rat-pancreatic-beta-celTranscription profiling of rat pancreatic beta-cells in response to double stranded RNA and IFNgamma exposureJan.08, 2015Viral infections and local production of IFNgamma might contribute to beta-cell dysfunction/death in Type 1 diabetes. Double stranded RNA accumulates in the cytosol of viral-infected cells, and exposure of purified rat beta cells to dsRNA (polyinosinic-polycytidylic acid, PIC) in combination with IFNgamma results in beta-cell dysfunction and apoptosis. To elucidate the molecular mechanisms involved in PIC + IFNgamma-effects, we determined the global profile of genes modified by these agents in primary rat beta cells. FACS-purified rat beta cells were cultured for 6 or 24 h in control condition or with IFNgamma, PIC or a combination of both agents. The gene expression profile was analyzed in duplicate with the Affymetrix RG U34A microarray.http://www.ebi.ac.uk/arrayexpress/experiments/E-CBIL-11rathttp://www.ebi.ac.uk/arrayexpress/experiments/E-CBIL-11/samples/