Dataset: Transcription profiling of rat pancreatic beta-cells in response to double stranded RNA and IFNgamma exposure
Viral infections and local production of IFNgamma might contribute to beta-cell dysfunction/death in Type 1 diabetes. Double stranded RNA...
Viral infections and local production of IFNgamma might contribute to beta-cell dysfunction/death in Type 1 diabetes. Double stranded RNA accumulates in the cytosol of viral-infected cells, and exposure of purified rat beta cells to dsRNA (polyinosinic-polycytidylic acid, PIC) in combination with IFNgamma results in beta-cell dysfunction and apoptosis. To elucidate the molecular mechanisms involved in PIC + IFNgamma-effects, we determined the global profile of genes modified by these agents in primary rat beta cells. FACS-purified rat beta cells were cultured for 6 or 24 h in control condition or with IFNgamma, PIC or a combination of both agents. The gene expression profile was analyzed in duplicate with the Affymetrix RG U34A microarray.
- Species:
- rat
- Samples:
- 62
- Source:
- E-CBIL-11
- PubMed:
- 14600816
- Updated:
- Feb.09, 2015
- Registered:
- Jan.08, 2015
| Sample | IFNgamma Concentration | PIC Concentration | Exposure Time |
|---|---|---|---|
| Wistar Rat 22 | 1,000,000 | 0.0 | 24 |
| Wistar Rat 22 | 1,000,000 | 0.0 | 24 |
| Wistar Rat 22 | 1,000,000 | 0.0 | 24 |
| Wistar Rat 22 | 1,000,000 | 0.0 | 24 |
| Wistar Rat 22 | 1,000,000 | 0.0 | 24 |
| Wistar Rat 22 | 1,000,000 | 0.0 | 24 |
| Wistar Rat 22 | 1,000,000 | 0.0 | 24 |
| Wistar Rat 22 | 1,000,000 | 0.0 | 24 |
| Wistar Rat 7 | 1,000,000 | 0.0 | 6 |
| Wistar Rat 7 | 1,000,000 | 0.0 | 6 |
| Wistar Rat 7 | 1,000,000 | 0.0 | 6 |
| Wistar Rat 7 | 1,000,000 | 0.0 | 6 |
| Wistar Rat 7 | 1,000,000 | 0.0 | 6 |
| Wistar Rat 7 | 1,000,000 | 0.0 | 6 |
| Wistar Rat 7 | 1,000,000 | 0.0 | 6 |
| Wistar Rat 7 | 1,000,000 | 0.0 | 6 |
| Wistar Rat 29 | 1,000,000 | 0.1 | 24 |
| Wistar Rat 29 | 1,000,000 | 0.1 | 24 |
| Wistar Rat 29 | 1,000,000 | 0.1 | 24 |
| Wistar Rat 29 | 1,000,000 | 0.1 | 24 |
| Wistar Rat 29 | 1,000,000 | 0.1 | 24 |
| Wistar Rat 29 | 1,000,000 | 0.1 | 24 |
| Wistar Rat 29 | 1,000,000 | 0.1 | 24 |
| Wistar Rat 29 | 1,000,000 | 0.1 | 24 |
| Wistar Rat 14 | 1,000,000 | 0.1 | 6 |
| Wistar Rat 14 | 1,000,000 | 0.1 | 6 |
| Wistar Rat 14 | 1,000,000 | 0.1 | 6 |
| Wistar Rat 14 | 1,000,000 | 0.1 | 6 |
| Wistar Rat 14 | 1,000,000 | 0.1 | 6 |
| Wistar Rat 14 | 1,000,000 | 0.1 | 6 |
| Wistar Rat 26 | 0 | 0.1 | 24 |
| Wistar Rat 26 | 0 | 0.1 | 24 |
| Wistar Rat 26 | 0 | 0.1 | 24 |
| Wistar Rat 26 | 0 | 0.1 | 24 |
| Wistar Rat 26 | 0 | 0.1 | 24 |
| Wistar Rat 26 | 0 | 0.1 | 24 |
| Wistar Rat 26 | 0 | 0.1 | 24 |
| Wistar Rat 26 | 0 | 0.1 | 24 |
| Wistar Rat 9 | 0 | 0.1 | 6 |
| Wistar Rat 9 | 0 | 0.1 | 6 |
| Wistar Rat 9 | 0 | 0.1 | 6 |
| Wistar Rat 9 | 0 | 0.1 | 6 |
| Wistar Rat 9 | 0 | 0.1 | 6 |
| Wistar Rat 9 | 0 | 0.1 | 6 |
| Wistar Rat 9 | 0 | 0.1 | 6 |
| Wistar Rat 9 | 0 | 0.1 | 6 |
| Wistar Rat 20 | 0.0 | 24 | |
| Wistar Rat 20 | 0.0 | 24 | |
| Wistar Rat 20 | 0.0 | 24 | |
| Wistar Rat 20 | 0.0 | 24 | |
| Wistar Rat 20 | 0.0 | 24 | |
| Wistar Rat 20 | 0.0 | 24 | |
| Wistar Rat 20 | 0.0 | 24 | |
| Wistar Rat 20 | 0.0 | 24 | |
| Wistar Rat 2 | 0.0 | 6 | |
| Wistar Rat 2 | 0.0 | 6 | |
| Wistar Rat 2 | 0.0 | 6 | |
| Wistar Rat 2 | 0.0 | 6 | |
| Wistar Rat 2 | 0.0 | 6 | |
| Wistar Rat 2 | 0.0 | 6 | |
| Wistar Rat 2 | 0.0 | 6 | |
| Wistar Rat 2 | 0.0 | 6 |