Dataset: The Complex Exogenous RNA Spectra in Human Plasma: An Interface with Human Gut Biota?

Human plasma has long been a rich source for biomarker discovery. It has recently become clear that plasma RNA molecules, such as...

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Human plasma has long been a rich source for biomarker discovery. It has recently become clear that plasma RNA molecules, such as microRNA, in addition to proteins are common and can serve as biomarkers. Surveying human plasma for microRNA biomarkers using next generation sequencing technology, we observed that a significant fraction of the circulating RNA appear to originate from exogenous species. With careful analysis of sequence error statistics and other controls, we demonstrated that there is a wide range of RNA from many different organisms, including bacteria and fungi as well as from other species. These RNAs may be associated with protein, lipid or other molecules protecting them from RNase activity in plasma. Some of these RNAs are detected in intracellular complexes and may be able to influence cellular activities under in vitro conditions. These findings raise the possibility that plasma RNAs of exogenous origin may serve as signaling molecules mediating for example the human-microbiome interaction and may affect and/or indicate the state of human health.

Species:: human
Samples:: 9
PubMed:: 23251414
Updated:: Jul.30, 2018
Registered:: Jul.30, 2018

Factors:

Sample	trancriptome_genome-ratio	cell_culture_source	related_dataset_id	fastq_identifier	anatomical_location	biosample_metadata_id	exRNA_source	transcriptome_reads	reference_genome_reads	biofluid_name	RNA_isolation_kit	profiling_assay	meets_ERCC_QC	species	condition
D3142P	0.460801	Not Applicable	EXR-DGALA1V5h5va-AN	sample_SM11_norm1_sequence_fastq	Entire cardiovascular system	EXR-DGALA1D3142PFN-BS	total cell-free biofluid RNA	230,891	501,064	Plasma	miRNeasy (Qiagen)	small RNA-Seq	NO	Human	Healthy Control
D3176P	0.357747	Not Applicable	EXR-DGALA1V5h5va-AN	sample_SM12_norm2_sequence_fastq	Entire cardiovascular system	EXR-DGALA1D3176PFN-BS	total cell-free biofluid RNA	142,222	397,549	Plasma	miRNeasy (Qiagen)	small RNA-Seq	NO	Human	Healthy Control
D3340P	0.382272	Not Applicable	EXR-DGALA1V5h5va-AN	sample_SM13_norm3_sequence_fastq	Entire cardiovascular system	EXR-DGALA1D3340PMN-BS	total cell-free biofluid RNA	155,011	405,499	Plasma	miRNeasy (Qiagen)	small RNA-Seq	NO	Human	Healthy Control
022163P	0.33314	Not Applicable	EXR-DGALA1V5h5va-AN	sample_SM1_crc1_sequence_fastq	Entire cardiovascular system	EXR-DGALA1022163PF-BS	total cell-free biofluid RNA	29,923	89,821	Plasma	miRNeasy (Qiagen)	small RNA-Seq	NO	Human	colorectal cancer
022273P	0.418862	Not Applicable	EXR-DGALA1V5h5va-AN	sample_SM2_crc2_sequence_fastq	Entire cardiovascular system	EXR-DGALA1022273PF-BS	total cell-free biofluid RNA	80,212	191,500	Plasma	miRNeasy (Qiagen)	small RNA-Seq	NO	Human	colorectal cancer
022299P	0.379583	Not Applicable	EXR-DGALA1V5h5va-AN	sample_SM3_crc3_sequence_fastq	Entire cardiovascular system	EXR-DGALA1022299PM-BS	total cell-free biofluid RNA	88,709	233,701	Plasma	miRNeasy (Qiagen)	small RNA-Seq	NO	Human	colorectal cancer
93163P	0.285972	Not Applicable	EXR-DGALA1V5h5va-AN	sample_SM6_uc1_sequence_fastq	Entire cardiovascular system	EXR-DGALA193163PMC-BS	total cell-free biofluid RNA	52,249	182,707	Plasma	miRNeasy (Qiagen)	small RNA-Seq	NO	Human	Ulcerative Colitis
93164P	0.268383	Not Applicable	EXR-DGALA1V5h5va-AN	sample_SM7_uc2_sequence_fastq	Entire cardiovascular system	EXR-DGALA193164PMC-BS	total cell-free biofluid RNA	50,522	188,246	Plasma	miRNeasy (Qiagen)	small RNA-Seq	NO	Human	Ulcerative Colitis
93166P	0.516342	Not Applicable	EXR-DGALA1V5h5va-AN	sample_SM8_uc3_sequence_fastq	Entire cardiovascular system	EXR-DGALA193166PFC-BS	total cell-free biofluid RNA	199,026	385,454	Plasma	miRNeasy (Qiagen)	small RNA-Seq	YES	Human	Ulcerative Colitis