Dataset: The Complex Exogenous RNA Spectra in Human Plasma: An Interface with Human Gut Biota?

Human plasma has long been a rich source for biomarker discovery. It has recently become clear that plasma RNA molecules, such as...

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Human plasma has long been a rich source for biomarker discovery. It has recently become clear that plasma RNA molecules, such as microRNA, in addition to proteins are common and can serve as biomarkers. Surveying human plasma for microRNA biomarkers using next generation sequencing technology, we observed that a significant fraction of the circulating RNA appear to originate from exogenous species. With careful analysis of sequence error statistics and other controls, we demonstrated that there is a wide range of RNA from many different organisms, including bacteria and fungi as well as from other species. These RNAs may be associated with protein, lipid or other molecules protecting them from RNase activity in plasma. Some of these RNAs are detected in intracellular complexes and may be able to influence cellular activities under in vitro conditions. These findings raise the possibility that plasma RNAs of exogenous origin may serve as signaling molecules mediating for example the human-microbiome interaction and may affect and/or indicate the state of human health.

Species:: human
Samples:: 9
PubMed:: 23251414
Updated:: Jul.30, 2018
Registered:: Jul.30, 2018

Factors:

Sample	trancriptome_genome-ratio	exRNA_source	related_dataset_id	fastq_identifier	biosample_metadata_id	condition	cell_culture_source	reference_genome_reads	transcriptome_reads	biofluid_name	RNA_isolation_kit	profiling_assay	meets_ERCC_QC	species	anatomical_location
D3142P	0.460801	total cell-free biofluid RNA	EXR-DGALA1V5h5va-AN	sample_SM11_norm1_sequence_fastq	EXR-DGALA1D3142PFN-BS	Healthy Control	Not Applicable	501,064	230,891	Plasma	miRNeasy (Qiagen)	small RNA-Seq	NO	Human	Entire cardiovascular system
D3176P	0.357747	total cell-free biofluid RNA	EXR-DGALA1V5h5va-AN	sample_SM12_norm2_sequence_fastq	EXR-DGALA1D3176PFN-BS	Healthy Control	Not Applicable	397,549	142,222	Plasma	miRNeasy (Qiagen)	small RNA-Seq	NO	Human	Entire cardiovascular system
D3340P	0.382272	total cell-free biofluid RNA	EXR-DGALA1V5h5va-AN	sample_SM13_norm3_sequence_fastq	EXR-DGALA1D3340PMN-BS	Healthy Control	Not Applicable	405,499	155,011	Plasma	miRNeasy (Qiagen)	small RNA-Seq	NO	Human	Entire cardiovascular system
022163P	0.33314	total cell-free biofluid RNA	EXR-DGALA1V5h5va-AN	sample_SM1_crc1_sequence_fastq	EXR-DGALA1022163PF-BS	colorectal cancer	Not Applicable	89,821	29,923	Plasma	miRNeasy (Qiagen)	small RNA-Seq	NO	Human	Entire cardiovascular system
022273P	0.418862	total cell-free biofluid RNA	EXR-DGALA1V5h5va-AN	sample_SM2_crc2_sequence_fastq	EXR-DGALA1022273PF-BS	colorectal cancer	Not Applicable	191,500	80,212	Plasma	miRNeasy (Qiagen)	small RNA-Seq	NO	Human	Entire cardiovascular system
022299P	0.379583	total cell-free biofluid RNA	EXR-DGALA1V5h5va-AN	sample_SM3_crc3_sequence_fastq	EXR-DGALA1022299PM-BS	colorectal cancer	Not Applicable	233,701	88,709	Plasma	miRNeasy (Qiagen)	small RNA-Seq	NO	Human	Entire cardiovascular system
93163P	0.285972	total cell-free biofluid RNA	EXR-DGALA1V5h5va-AN	sample_SM6_uc1_sequence_fastq	EXR-DGALA193163PMC-BS	Ulcerative Colitis	Not Applicable	182,707	52,249	Plasma	miRNeasy (Qiagen)	small RNA-Seq	NO	Human	Entire cardiovascular system
93164P	0.268383	total cell-free biofluid RNA	EXR-DGALA1V5h5va-AN	sample_SM7_uc2_sequence_fastq	EXR-DGALA193164PMC-BS	Ulcerative Colitis	Not Applicable	188,246	50,522	Plasma	miRNeasy (Qiagen)	small RNA-Seq	NO	Human	Entire cardiovascular system
93166P	0.516342	total cell-free biofluid RNA	EXR-DGALA1V5h5va-AN	sample_SM8_uc3_sequence_fastq	EXR-DGALA193166PFC-BS	Ulcerative Colitis	Not Applicable	385,454	199,026	Plasma	miRNeasy (Qiagen)	small RNA-Seq	YES	Human	Entire cardiovascular system