ERCC0100490.9735726,198,668EXR-SADAS1EXER1-ANsample_Sample_003_EX_BL_CAGATC_R1_001_fastqEXR-SADAS1Ex0301-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES6,366,933Healthy ControlPlasma0.9463576,385,726EXR-SADAS1EXER1-ANsample_Sample_003_EX_PK_CAACTA_R1_001_fastqEXR-SADAS1Ex0302-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES6,747,691Healthy ControlPlasma0.9911637,005,992EXR-SADAS1EXER1-ANsample_Sample_004_EX_BL_TAGCTT_R1_001_fastqEXR-SADAS1Ex0401-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES7,068,457Healthy ControlPlasma0.9252574,763,934EXR-SADAS1EXER1-ANsample_Sample_004_EX_PK_CGGAAT_R1_001_fastqEXR-SADAS1Ex0402-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES5,148,767Healthy ControlPlasma0.9823026,565,482EXR-SADAS1EXER1-ANsample_Sample_006_EX_BL_ACTTGA_R1_001_fastqEXR-SADAS1Ex0601-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES6,683,771Healthy ControlPlasma0.9612734,691,395EXR-SADAS1EXER1-ANsample_Sample_006_EX_PK_CAGGCG_R1_001_fastqEXR-SADAS1Ex0602-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES4,880,400Healthy ControlPlasma0.9665375,040,038EXR-SADAS1EXER1-ANsample_Sample_007_EX_BL_ATCACG_R1_001_fastqEXR-SADAS1Ex0701-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES5,214,531Healthy ControlPlasma0.9544224,000,090EXR-SADAS1EXER1-ANsample_Sample_007_EX_PK_GGCTAC_R1_001_fastqEXR-SADAS1Ex0702-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES4,191,113Healthy ControlPlasma0.9759996,184,812EXR-SADAS1EXER1-ANsample_Sample_010_EX_BL_CTTGTA_R1_001_fastqEXR-SADAS1Ex1001-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES6,336,905Healthy ControlPlasma0.927144,154,267EXR-SADAS1EXER1-ANsample_Sample_010_EX_PK_CAAAAG_R1_001_fastqEXR-SADAS1Ex1002-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES4,480,735Healthy ControlPlasma0.673709393,397EXR-SADAS1EXER1-ANsample_Sample_012_EX_BL_ACTGAT_combined_2_R1_001_fastqEXR-SADAS1Ex1201-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES583,927Healthy ControlPlasma0.9598074,298,537EXR-SADAS1EXER1-ANsample_Sample_012_EX_PK_CACTCA_R1_001_fastqEXR-SADAS1Ex1202-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES4,478,541Healthy ControlPlasma0.903039971,498EXR-SADAS1EXER1-ANsample_Sample_013_EX_BL_GAGTGG_R1_001_fastqEXR-SADAS1Ex1301-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES1,075,810Healthy ControlPlasma0.9252322,729,074EXR-SADAS1EXER1-ANsample_Sample_013_EX_PK_ATTCCT_R1_001_fastqEXR-SADAS1Ex1302-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES2,949,612Healthy ControlPlasma0.9706924,240,675EXR-SADAS1EXER1-ANsample_Sample_014_EX_BL_ACAGTG_R1_001_fastqEXR-SADAS1Ex1401-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES4,368,712Healthy ControlPlasma0.9583125,727,496EXR-SADAS1EXER1-ANsample_Sample_014_EX_PK_GTAGAG_R1_001_fastqEXR-SADAS1Ex1402-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES5,976,651Healthy ControlPlasma0.288149138,797EXR-SADAS1EXER1-ANsample_Sample_018_EX_BL_GTGAAA_combined_2_R1_001_fastqEXR-SADAS1Ex1801-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqNO481,685Healthy ControlPlasma0.9764544,489,788EXR-SADAS1EXER1-ANsample_Sample_018_EX_PK_TTAGGC_R1_001_fastqEXR-SADAS1Ex1802-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES4,598,055Healthy ControlPlasma0.9702724,126,161EXR-SADAS1EXER1-ANsample_Sample_019_EX_BL_GATCAG_R1_001_fastqEXR-SADAS1Ex1901-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES4,252,581Healthy ControlPlasma0.9489373,921,983EXR-SADAS1EXER1-ANsample_Sample_019_EX_PK_ATGAGC_R1_001_fastqEXR-SADAS1Ex1902-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES4,133,029Healthy ControlPlasma0.9926864,969,606EXR-SADAS1EXER1-ANsample_Sample_023_EX_BL_GTTTCG_R1_001_fastqEXR-SADAS1Ex2301-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES5,006,223Healthy ControlPlasma0.9776035,213,865EXR-SADAS1EXER1-ANsample_Sample_023_EX_PK_TGACCA_R1_001_fastqEXR-SADAS1Ex2302-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES5,333,315Healthy ControlPlasma0.851699861,043EXR-SADAS1EXER1-ANsample_Sample_024_EX_BL_AGTCAA_combined_2_R1_001_fastqEXR-SADAS1Ex2401-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES1,010,971Healthy ControlPlasma0.9100073,333,574EXR-SADAS1EXER1-ANsample_Sample_024_EX_PK_CTATAC_R1_001_fastqEXR-SADAS1Ex2402-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES3,663,238Healthy ControlPlasma0.8865691,122,167EXR-SADAS1EXER1-ANsample_Sample_025_EX_BL_GGTAGC_R1_001_fastqEXR-SADAS1Ex2501-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES1,265,742Healthy ControlPlasma0.8661963,551,632EXR-SADAS1EXER1-ANsample_Sample_025_EX_PK_CCGTCC_R1_001_fastqEXR-SADAS1Ex2502-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES4,100,266Healthy ControlPlasma0.9818796,616,604EXR-SADAS1EXER1-ANsample_Sample_026_EX_BL_GCCAAT_R1_001_fastqEXR-SADAS1Ex2601-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES6,738,716Healthy ControlPlasma0.6226452,513,733EXR-SADAS1EXER1-ANsample_Sample_026_EX_PK_CATGGC_combined_2_R1_001_fastqEXR-SADAS1Ex2602-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES4,037,185Healthy ControlPlasma0.9649375,421,905EXR-SADAS1EXER1-ANsample_Sample_027_EX_BL_GTGGCC_R1_001_fastqEXR-SADAS1Ex2701-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES5,618,924Healthy ControlPlasma0.9274457,468,854EXR-SADAS1EXER1-ANsample_Sample_027_EX_PK_CACGAT_R1_001_fastqEXR-SADAS1Ex2702-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES8,053,154Healthy ControlPlasma0.9737646,229,620EXR-SADAS1EXER1-ANsample_Sample_028_EX_BL_AGTTCC_R1_001_fastqEXR-SADAS1Ex2801-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES6,397,463Healthy ControlPlasma0.9763878,112,878EXR-SADAS1EXER1-ANsample_Sample_028_EX_PK_CTCAGA_R1_001_fastqEXR-SADAS1Ex2802-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES8,309,078Healthy ControlPlasma0.9460252,650,656EXR-SADAS1EXER1-ANsample_Sample_029_EX_BL_CGATGT_R1_001_fastqEXR-SADAS1Ex2901-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES2,801,887Healthy ControlPlasma0.9320214,921,196EXR-SADAS1EXER1-ANsample_Sample_029_EX_PK_CTAGCT_R1_001_fastqEXR-SADAS1Ex2902-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES5,280,133Healthy ControlPlasma0.617294371,861EXR-SADAS1EXER1-ANsample_Sample_030_EX_BL_CGTACG_combined_2_R1_001_fastqEXR-SADAS1Ex3001-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES602,405Healthy ControlPlasma0.8643683,281,101EXR-SADAS1EXER1-ANsample_Sample_030_EX_PK_GACGAC_R1_001_fastqEXR-SADAS1Ex3002-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES3,795,956Healthy ControlPlasma0.9553714,536,552EXR-SADAS1EXER1-ANsample_Sample_031_EX_BL_GTCCGC_R1_001_fastqEXR-SADAS1Ex3101-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES4,748,474Healthy ControlPlasma0.9567995,450,772EXR-SADAS1EXER1-ANsample_Sample_031_EX_PK_CCAACA_R1_001_fastqEXR-SADAS1Ex3102-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES5,696,881Healthy ControlPlasma0.9874618,627,840EXR-SADAS1EXER1-ANsample_Sample_032_EX_BL_ATGTCA_R1_001_fastqEXR-SADAS1Ex3201-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES8,737,396Healthy ControlPlasma0.9639355,865,851EXR-SADAS1EXER1-ANsample_Sample_032_EX_PK_CATTTT_R1_001_fastqEXR-SADAS1Ex3202-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES6,085,317Healthy ControlPlasma0.9156193,558,844EXR-SADAS1EXER1-ANsample_Sample_044_BL_Vs_ATCACG_R1_001_fastqEXR-SADAS1Ex4401-BSextracellular vesicleNot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES3,886,818Healthy ControlPlasma0.9882345,783,466EXR-SADAS1EXER1-ANsample_Sample_044_Ex_BL_GGCTAC_R1_001_fastqEXR-SADAS1Ex4402-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES5,852,322Healthy ControlPlasma0.9773183,341,569EXR-SADAS1EXER1-ANsample_Sample_044_Ex_PK_CCGTCC_R1_001_fastqEXR-SADAS1Ex4403-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES3,419,123Healthy ControlPlasma0.50682849,163EXR-SADAS1EXER1-ANsample_Sample_044_PK_Vs_GCCAAT_combined_2_R1_001_fastqEXR-SADAS1Ex4404-BSextracellular vesicleNot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES1,675,473Healthy ControlPlasma0.9969794,729,657EXR-SADAS1EXER1-ANsample_Sample_059_EX_BL_CACCGG_R1_001_fastqEXR-SADAS1Ex5901-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES4,743,989Healthy ControlPlasma0.99739810,941,804EXR-SADAS1EXER1-ANsample_Sample_059_EX_PK_TAATCG_combined_2_R1_001_fastqEXR-SADAS1Ex5902-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES10,970,346Healthy ControlPlasma0.475969770,280EXR-SADAS1EXER1-ANsample_Sample_068_BL_Vs_CGATGT_combined_2_R1_001_fastqEXR-SADAS1Ex6801-BSextracellular vesicleNot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqNO1,618,341Healthy ControlPlasma0.99571410,070,795EXR-SADAS1EXER1-ANsample_Sample_068_Ex_BL_CTTGTA_R1_001_fastqEXR-SADAS1Ex6802-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES10,114,141Healthy ControlPlasma0.9426211,590,435EXR-SADAS1EXER1-ANsample_Sample_068_Ex_PK_GTAGAG_R1_001_fastqEXR-SADAS1Ex6803-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES1,687,248Healthy ControlPlasma0.469104199,597EXR-SADAS1EXER1-ANsample_Sample_068_PK_Vs_CAGATC_R1_001_fastqEXR-SADAS1Ex6804-BSextracellular vesicleNot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqNO425,486Healthy ControlPlasma0.8441062,414,319EXR-SADAS1EXER1-ANsample_Sample_070_BL_Vs_TTAGGC_R1_001_fastqEXR-SADAS1Ex7001-BSextracellular vesicleNot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES2,860,210Healthy ControlPlasma0.9800846,399,067EXR-SADAS1EXER1-ANsample_Sample_070_Ex_BL_AGTCAA_R1_001_fastqEXR-SADAS1Ex7002-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES6,529,102Healthy ControlPlasma0.9738771,974,718EXR-SADAS1EXER1-ANsample_Sample_070_Ex_PK_GTCCGC_R1_001_fastqEXR-SADAS1Ex7003-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES2,027,688Healthy ControlPlasma0.8385422,198,977EXR-SADAS1EXER1-ANsample_Sample_070_PK_Vs_ACTTGA_R1_001_fastqEXR-SADAS1Ex7004-BSextracellular vesicleNot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES2,622,380Healthy ControlPlasma0.5661071,000,344EXR-SADAS1EXER1-ANsample_Sample_077_BL_Vs_TGACCA_combined_2_R1_001_fastqEXR-SADAS1Ex7701-BSextracellular vesicleNot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES1,767,058Healthy ControlPlasma0.9708692,509,513EXR-SADAS1EXER1-ANsample_Sample_077_Ex_BL_AGTTCC_R1_001_fastqEXR-SADAS1Ex7702-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES2,584,812Healthy ControlPlasma0.9499084,606,619EXR-SADAS1EXER1-ANsample_Sample_077_Ex_PK_GTGAAA_R1_001_fastqEXR-SADAS1Ex7703-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES4,849,542Healthy ControlPlasma0.378053333,378EXR-SADAS1EXER1-ANsample_Sample_077_PK_Vs_GATCAG_combined_2_R1_001_fastqEXR-SADAS1Ex7704-BSextracellular vesicleNot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqNO881,828Healthy ControlPlasma0.912513,901,117EXR-SADAS1EXER1-ANsample_Sample_078_BL_Vs_ACAGTG_R1_001_fastqEXR-SADAS1Ex7801-BSextracellular vesicleNot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES4,275,152Healthy ControlPlasma0.9885648,027,788EXR-SADAS1EXER1-ANsample_Sample_078_Ex_BL_ATGTCA_R1_001_fastqEXR-SADAS1Ex7802-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES8,120,656Healthy ControlPlasma0.9798132,127,188EXR-SADAS1EXER1-ANsample_Sample_078_Ex_PK_GTGGCC_R1_001_fastqEXR-SADAS1Ex7803-BStotal cell-free biofluid RNANot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES2,171,015Healthy ControlPlasma0.9684055,086,697EXR-SADAS1EXER1-ANsample_Sample_078_PK_Vs_TAGCTT_R1_001_fastqEXR-SADAS1Ex7804-BSextracellular vesicleNot ApplicableHumanEntire body systemMiRVana Paris (Ambion)small RNA-SeqYES5,252,655Healthy ControlPlasmaunknown21Exercise improves cardiometabolic and vascular function, though mechanisms remain unclear. Our objective was to demonstrate the diversity...28916639/profile/10501/ercc1562100423015Jul.30, 2018Falseunknownsmall-rna-seq-during-acute-maximal-exercise-revealunknownSmall RNA-seq during acute maximal exercise reveal RNAs involved in vascular inflammation and cardiometabolic healthJul.30, 2018Exercise improves cardiometabolic and vascular function, though mechanisms remain unclear. Our objective was to demonstrate the diversity of circulating extracellular RNA (ex-RNA) release during acute exercise in humans and its relevance to exercise-mediated benefits on vascular inflammation. We performed plasma small RNA sequencing (RNA-seq) in 26 individuals undergoing symptom-limited maximal treadmill exercise, with replication of our top candidate miRNA in a separate cohort of 59 individuals undergoing bicycle ergometry. We found changes in miRNAs and other ex-RNAs with exercise (e.g., y-RNAs, t-RNAs) implicated in cardiovascular disease. In two independent cohorts of acute maximal exercise, we identified miR-181b-5p as a key ex-RNA increased in plasma after exercise, with validation in a separate cohort. In a mouse model of acute exercise, we found significant increases in miR-181b-5p expression in skeletal muscle after acute exercise in young (but not older) mice. Previous work revealed a strong role for miR-181b-5p in vascular inflammation in obesity, insulin resistance, sepsis, and cardiovascular disease. Circulating ex-RNAs altered in plasma after acute exercise target pathways involved in inflammation, including miR-181b-5p. Further investigation into the role of known (e.g., miRNA) and novel (e.g., y-RNAs) is warranted to uncover new mechanisms of vascular inflammation on exercise-mediated benefits on health.BDS_00038human